EC Number | Cloned (Comment) | Organism |
---|---|---|
1.17.4.1 | expression of wild-type and amino terminal deleted DELTA1-248 R1 subunit and wild-type, F127Y, Y129F and F127Y/Y129F mutant R2 subunit in Escherichia coli | Chlamydia trachomatis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.17.4.1 | F127Y | similar CDP reductase activity as wild-type | Chlamydia trachomatis |
1.17.4.1 | F127Y/Y129F | 10-15% of wild-type CDP reductase activity | Chlamydia trachomatis |
1.17.4.1 | Y129F | no CDP reductase activity | Chlamydia trachomatis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.1 | Hydroxyurea | - |
Chlamydia trachomatis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.4.1 | Mg2+ | - |
Chlamydia trachomatis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.4.1 | Chlamydia trachomatis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.4.1 | recombinant wild-type R1 and R2 subunits and F127Y, Y129F and F127Y/Y129F R2 mutant subunits | Chlamydia trachomatis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.4.1 | 0.035 | - |
recombinant R1 subunit, CDP reduction in the presence of ATP | Chlamydia trachomatis |
1.17.4.1 | 0.048 | - |
recombinant DELTA1-248 R1 subunit, CDP reduction in the presence of ATP | Chlamydia trachomatis |
1.17.4.1 | 0.18 | - |
recombinant R2 subunit, CDP reduction in the presence of ATP | Chlamydia trachomatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.4.1 | ribonucleoside diphosphate + reduced thioredoxin | - |
Chlamydia trachomatis | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |