BRENDA - Enzyme Database

The manganese-containing ribonucleotide reductase of Corynebacterium ammoniagenes is a class Ib enzyme

Fieschi, F.; Torrents, E.; Toulokhonova, L.; Jordan, A.; Hellman, U.; Barbe, J.; Gibert, I.; Karlsson, M.; Sjoberg, B.M.; J. Biol. Chem. 273, 4329-4337 (1998)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
ATP
stimulation of CDP reduction
Corynebacterium ammoniagenes
1.17.4.1
dATP
stimulation of CDP reduction
Corynebacterium ammoniagenes
1.17.4.1
dTTP
stimulation of CDP reduction
Corynebacterium ammoniagenes
Cloned(Commentary)
EC Number
Commentary
Organism
1.17.4.1
expression R1E and R2F subunits in Escherichia coli
Corynebacterium ammoniagenes
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
Hydroxyurea
2 mM, approx. 80% inactivation
Corynebacterium ammoniagenes
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.4.1
Mn2+
EPR-silent Mn bound to the polypeptide chain, approx. 0.5 mol manganese ions/mol of R2F polypeptide
Corynebacterium ammoniagenes
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.4.1
37900
-
alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence
Corynebacterium ammoniagenes
1.17.4.1
81200
-
alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence
Corynebacterium ammoniagenes
1.17.4.1
160000
-
gel filtration
Corynebacterium ammoniagenes
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.4.1
Corynebacterium ammoniagenes
O69273
class Ib reductase
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.4.1
low-salt precipitation, DEAE-cellulose, Supredex 200, Mono Q
Corynebacterium ammoniagenes
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.17.4.1
0.034
-
-
Corynebacterium ammoniagenes
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
NrdH-redoxin obtained from an overproducing strain, no activity with E. coli thioredoxin
437981
Corynebacterium ammoniagenes
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437981
Corynebacterium ammoniagenes
ir
Subunits
EC Number
Subunits
Commentary
Organism
1.17.4.1
trimer
alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence
Corynebacterium ammoniagenes
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.17.4.1
ATP
stimulation of CDP reduction
Corynebacterium ammoniagenes
1.17.4.1
dATP
stimulation of CDP reduction
Corynebacterium ammoniagenes
1.17.4.1
dTTP
stimulation of CDP reduction
Corynebacterium ammoniagenes
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.4.1
expression R1E and R2F subunits in Escherichia coli
Corynebacterium ammoniagenes
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.4.1
Hydroxyurea
2 mM, approx. 80% inactivation
Corynebacterium ammoniagenes
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.17.4.1
Mn2+
EPR-silent Mn bound to the polypeptide chain, approx. 0.5 mol manganese ions/mol of R2F polypeptide
Corynebacterium ammoniagenes
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.4.1
37900
-
alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence
Corynebacterium ammoniagenes
1.17.4.1
81200
-
alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence
Corynebacterium ammoniagenes
1.17.4.1
160000
-
gel filtration
Corynebacterium ammoniagenes
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.4.1
low-salt precipitation, DEAE-cellulose, Supredex 200, Mono Q
Corynebacterium ammoniagenes
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.17.4.1
0.034
-
-
Corynebacterium ammoniagenes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.4.1
ribonucleoside diphosphate + reduced thioredoxin
NrdH-redoxin obtained from an overproducing strain, no activity with E. coli thioredoxin
437981
Corynebacterium ammoniagenes
2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
437981
Corynebacterium ammoniagenes
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.4.1
trimer
alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence
Corynebacterium ammoniagenes