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Literature summary extracted from

  • Berglund, O.
    Ribonucleoside diphosphate reductase induced by bacteriophage Tr. I. Purification and characterization (1972), J. Biol. Chem., 247, 7270-7275.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 E. coli thioredoxin reductase absolute requirement Tequatrovirus T4
1.17.4.1 E. coli thioredoxin reductase enzyme induced in Escherichia coli by infection with bacteriophage T4 Tequatrovirus T4
1.17.4.1 thioredoxin reductase Escherichia coli enzyme Tequatrovirus T4

General Stability

EC Number General Stability Organism
1.17.4.1 dithiothreitol, 1 mM, stabilizes Tequatrovirus T4
1.17.4.1 protein concentrations above 5 mg/ml stabilizes Tequatrovirus T4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.1 additional information
-
 additional information effect of different nucleoside triphosphates on Km Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.1 Iron 2.3 atoms of nonheme iron per molecule Tequatrovirus T4
1.17.4.1 Mg2+ 5-10 mM, 2-3fold stimulation, not required for enzyme activity Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.1 35000
-
 alpha2beta2, 2 * 85000 + 2 * 35000, enzyme induced in Escherichia coli after infection with bacteriophage T4, SDS-PAGE Tequatrovirus T4
1.17.4.1 85000
-
 alpha2beta2, 2 * 85000 + 2 * 35000, enzyme induced in Escherichia coli after infection with bacteriophage T4, SDS-PAGE Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Tequatrovirus T4
-
 enzyme induced in Escherichia coli by infection with bacteriophage T4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.1 enzyme induced in Escherichia coli after infection with bacteriophage T4, streptomycin, ammonium sulfate, dATP-Sepharose Tequatrovirus T4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.1 1.09
-
-
 Tequatrovirus T4

Storage Stability

EC Number Storage Stability Organism
1.17.4.1 -70°C, 100 mM potassium phosphate, pH 7.0, 1 mM dithiothreitol, enzyme concentration 4.8 mg/ml, 6 months, no loss of activity Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 ribonucleoside diphosphate + reduced thioredoxin dithiothreitol at higher concentrations i.e. 100 mM can partially substitute for reduced T4 thioredoxin, the rate of CDP reduction is 10% of that obtained with the complete system Tequatrovirus T4 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir

Subunits

EC Number Subunits Comment Organism
1.17.4.1 tetramer alpha2beta2, 2 * 85000 + 2 * 35000, enzyme induced in Escherichia coli after infection with bacteriophage T4, SDS-PAGE Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.1 7 8
-
 Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 NADPH absolute requirement Tequatrovirus T4
1.17.4.1 T4 thioredoxin absolute requirement Tequatrovirus T4
1.17.4.1 T4 thioredoxin enzyme induced in E. coli by infection with bacteriophage T4 Tequatrovirus T4