1.17.9.1

effect of pH-value and ionic strength on KM-value

Pseudomonas putida

1.17.9.1

0.0264

phenazine methosulfate

reaction with 4-n-propylphenol

Pseudomonas putida

1.17.9.1

0.366

phenazine methosulfate

reaction with 2-bromo-4-methylphenol

Pseudomonas putida

1.17.9.1

1.94

phenazine methosulfate

reaction with 4-ethylphenol

Pseudomonas putida

1.17.9.1

3.26

phenazine methosulfate

reaction with 4-hydroxybenzyl alcohol

Pseudomonas putida

1.17.9.1

5.23

phenazine methosulfate

reaction with 2,4-dimethylphenol

Pseudomonas putida

1.17.9.1

6.76

phenazine methosulfate

reaction with 4-cresol

Pseudomonas putida

1.17.9.1

Pseudomonas putida

1.17.9.1

(RS)-1-(4-hydroxyphenyl)ethanol + acceptor + H2O

437874

Pseudomonas putida

?

?

1.17.9.1

1,4-hydroxyquinone + acceptor + H2O

437874

Pseudomonas putida

?

?

1.17.9.1

2,4-xylenol + acceptor + H2O

i.e. 2,4-dimethylphenol

437874

Pseudomonas putida

3-methyl-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

2-bromo-4-methylphenol + acceptor + H2O

437874

Pseudomonas putida

3-bromo-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

2-methoxy-4-methylphenol + acceptor + H2O

437874

Pseudomonas putida

3-methoxy-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

3,4-dimethylphenol + acceptor + H2O

437874

Pseudomonas putida

2-methyl-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

3-fluoro-4-methylphenol + acceptor + H2O

437874

Pseudomonas putida

2-fluoro-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

4-cresol + acceptor + H2O

acceptor: phenazine methosulfate

437874

Pseudomonas putida

4-hydroxybenzaldehyde + reduced acceptor

437874

Pseudomonas putida

?

1.17.9.1

4-cresol + acceptor + H2O

no activity with 2,6-dichlorophenol-indophenol as acceptor

437874

Pseudomonas putida

4-hydroxybenzaldehyde + reduced acceptor

437874

Pseudomonas putida

?

1.17.9.1

4-cresol + acceptor + H2O

no activity with O2, K3Fe(CN)6 or methylene blue

437874

Pseudomonas putida

4-hydroxybenzaldehyde + reduced acceptor

437874

Pseudomonas putida

?

1.17.9.1

4-cresol + acceptor + H2O

the enzyme catalyzes both 4-cresol hydroxylation and further oxidation of the product, 4-hydroxybenzyl alcohol to 4-hydroxybenzaldehyde

437874

Pseudomonas putida

4-hydroxybenzaldehyde + reduced acceptor

437874

Pseudomonas putida

?

1.17.9.1

4-cresol + phenazine methosulfate + H2O

437874

Pseudomonas putida

4-hydroxybenzyl alcohol + reduced phenazine methosulfate

?

1.17.9.1

4-ethylphenol + acceptor + H2O

437874

Pseudomonas putida

1-(4'-hydroxyphenyl)ethanol + reduced acceptor

?

1.17.9.1

4-hydroxybenzyl alcohol + acceptor

437874

Pseudomonas putida

4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

4-isopropylphenol + acceptor + H2O

437874

Pseudomonas putida

?

?

1.17.9.1

4-methyl-1-naphthol + acceptor + H2O

437874

Pseudomonas putida

?

?

1.17.9.1

4-methyl-3-nitrophenol + acceptor + H2O

437874

Pseudomonas putida

2-nitro-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

4-methylcatechol + acceptor + H2O

437874

Pseudomonas putida

3,4-dihydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

4-n-propylphenol + acceptor + H2O

437874

Pseudomonas putida

?

?

1.17.9.1

no activity with 2-cresol

437874

Pseudomonas putida

?

?

1.17.9.1

no activity with 4-methyl-2-nitrophenol, 2,4,5-trimethylphenol, 2-chloro-4,5-dimethylphenol, 2,4,6-trimethylphenol, 2,6-dibromo-4-methylphenol, L-tyrosine, 4-hydroxybenzaldehyde 3-cresol

437874

Pseudomonas putida

?

?

1.17.9.1

8.3

Pseudomonas putida

1.17.9.1

cytochrome c

enzyme contains covalently bound flavin and a type c cytochrome

Pseudomonas putida

1.17.9.1

cytochrome c

enzyme contains covalently bound flavin and a type c cytochrome

Pseudomonas putida

1.17.9.1

effect of pH-value and ionic strength on KM-value

Pseudomonas putida

1.17.9.1

0.0264

phenazine methosulfate

reaction with 4-n-propylphenol

Pseudomonas putida

1.17.9.1

0.366

phenazine methosulfate

reaction with 2-bromo-4-methylphenol

Pseudomonas putida

1.17.9.1

1.94

phenazine methosulfate

reaction with 4-ethylphenol

Pseudomonas putida

1.17.9.1

3.26

phenazine methosulfate

reaction with 4-hydroxybenzyl alcohol

Pseudomonas putida

1.17.9.1

5.23

phenazine methosulfate

reaction with 2,4-dimethylphenol

Pseudomonas putida

1.17.9.1

6.76

phenazine methosulfate

reaction with 4-cresol

Pseudomonas putida

1.17.9.1

(RS)-1-(4-hydroxyphenyl)ethanol + acceptor + H2O

437874

Pseudomonas putida

?

?

1.17.9.1

1,4-hydroxyquinone + acceptor + H2O

437874

Pseudomonas putida

?

?

1.17.9.1

2,4-xylenol + acceptor + H2O

i.e. 2,4-dimethylphenol

437874

Pseudomonas putida

3-methyl-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

2-bromo-4-methylphenol + acceptor + H2O

437874

Pseudomonas putida

3-bromo-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

2-methoxy-4-methylphenol + acceptor + H2O

437874

Pseudomonas putida

3-methoxy-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

3,4-dimethylphenol + acceptor + H2O

437874

Pseudomonas putida

2-methyl-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

3-fluoro-4-methylphenol + acceptor + H2O

437874

Pseudomonas putida

2-fluoro-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

4-cresol + acceptor + H2O

acceptor: phenazine methosulfate

437874

Pseudomonas putida

4-hydroxybenzaldehyde + reduced acceptor

437874

Pseudomonas putida

?

1.17.9.1

4-cresol + acceptor + H2O

no activity with 2,6-dichlorophenol-indophenol as acceptor

437874

Pseudomonas putida

4-hydroxybenzaldehyde + reduced acceptor

437874

Pseudomonas putida

?

1.17.9.1

4-cresol + acceptor + H2O

no activity with O2, K3Fe(CN)6 or methylene blue

437874

Pseudomonas putida

4-hydroxybenzaldehyde + reduced acceptor

437874

Pseudomonas putida

?

1.17.9.1

4-cresol + acceptor + H2O

the enzyme catalyzes both 4-cresol hydroxylation and further oxidation of the product, 4-hydroxybenzyl alcohol to 4-hydroxybenzaldehyde

437874

Pseudomonas putida

4-hydroxybenzaldehyde + reduced acceptor

437874

Pseudomonas putida

?

1.17.9.1

4-cresol + phenazine methosulfate + H2O

437874

Pseudomonas putida

4-hydroxybenzyl alcohol + reduced phenazine methosulfate

?

1.17.9.1

4-ethylphenol + acceptor + H2O

437874

Pseudomonas putida

1-(4'-hydroxyphenyl)ethanol + reduced acceptor

?

1.17.9.1

4-hydroxybenzyl alcohol + acceptor

437874

Pseudomonas putida

4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

4-isopropylphenol + acceptor + H2O

437874

Pseudomonas putida

?

?

1.17.9.1

4-methyl-1-naphthol + acceptor + H2O

437874

Pseudomonas putida

?

?

1.17.9.1

4-methyl-3-nitrophenol + acceptor + H2O

437874

Pseudomonas putida

2-nitro-4-hydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

4-methylcatechol + acceptor + H2O

437874

Pseudomonas putida

3,4-dihydroxybenzaldehyde + reduced acceptor

?

1.17.9.1

4-n-propylphenol + acceptor + H2O

437874

Pseudomonas putida

?

?

1.17.9.1

no activity with 2-cresol

437874

Pseudomonas putida

?

?

1.17.9.1

no activity with 4-methyl-2-nitrophenol, 2,4,5-trimethylphenol, 2-chloro-4,5-dimethylphenol, 2,4,6-trimethylphenol, 2,6-dibromo-4-methylphenol, L-tyrosine, 4-hydroxybenzaldehyde 3-cresol

437874

Pseudomonas putida

?

?

1.17.9.1

8.3

Pseudomonas putida