BRENDA - Enzyme Database

p-Cresol methylhydroxylase. assay and general properties

McIntire, W.; Hopper, D.J.; Singer, T.P.; Biochem. J. 228, 325-335 (1985) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.9.1
additional information
-
additional information
effect of pH-value and ionic strength on KM-value
Pseudomonas putida
1.17.9.1
0.0264
-
phenazine methosulfate
reaction with 4-n-propylphenol
Pseudomonas putida
1.17.9.1
0.366
-
phenazine methosulfate
reaction with 2-bromo-4-methylphenol
Pseudomonas putida
1.17.9.1
1.94
-
phenazine methosulfate
reaction with 4-ethylphenol
Pseudomonas putida
1.17.9.1
3.26
-
phenazine methosulfate
reaction with 4-hydroxybenzyl alcohol
Pseudomonas putida
1.17.9.1
5.23
-
phenazine methosulfate
reaction with 2,4-dimethylphenol
Pseudomonas putida
1.17.9.1
6.76
-
phenazine methosulfate
reaction with 4-cresol
Pseudomonas putida
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.17.9.1
Pseudomonas putida
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.17.9.1
(RS)-1-(4-hydroxyphenyl)ethanol + acceptor + H2O
-
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
1,4-hydroxyquinone + acceptor + H2O
-
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
2,4-xylenol + acceptor + H2O
i.e. 2,4-dimethylphenol
437874
Pseudomonas putida
3-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
2-bromo-4-methylphenol + acceptor + H2O
-
437874
Pseudomonas putida
3-bromo-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
2-methoxy-4-methylphenol + acceptor + H2O
-
437874
Pseudomonas putida
3-methoxy-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
3,4-dimethylphenol + acceptor + H2O
-
437874
Pseudomonas putida
2-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
3-fluoro-4-methylphenol + acceptor + H2O
-
437874
Pseudomonas putida
2-fluoro-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
4-cresol + acceptor + H2O
acceptor: phenazine methosulfate
437874
Pseudomonas putida
4-hydroxybenzaldehyde + reduced acceptor
-
437874
Pseudomonas putida
?
1.17.9.1
4-cresol + acceptor + H2O
no activity with 2,6-dichlorophenol-indophenol as acceptor
437874
Pseudomonas putida
4-hydroxybenzaldehyde + reduced acceptor
-
437874
Pseudomonas putida
?
1.17.9.1
4-cresol + acceptor + H2O
no activity with O2, K3Fe(CN)6 or methylene blue
437874
Pseudomonas putida
4-hydroxybenzaldehyde + reduced acceptor
-
437874
Pseudomonas putida
?
1.17.9.1
4-cresol + acceptor + H2O
the enzyme catalyzes both 4-cresol hydroxylation and further oxidation of the product, 4-hydroxybenzyl alcohol to 4-hydroxybenzaldehyde
437874
Pseudomonas putida
4-hydroxybenzaldehyde + reduced acceptor
-
437874
Pseudomonas putida
?
1.17.9.1
4-cresol + phenazine methosulfate + H2O
-
437874
Pseudomonas putida
4-hydroxybenzyl alcohol + reduced phenazine methosulfate
-
-
-
?
1.17.9.1
4-ethylphenol + acceptor + H2O
-
437874
Pseudomonas putida
1-(4'-hydroxyphenyl)ethanol + reduced acceptor
-
-
-
?
1.17.9.1
4-hydroxybenzyl alcohol + acceptor
-
437874
Pseudomonas putida
4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
4-isopropylphenol + acceptor + H2O
-
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
4-methyl-1-naphthol + acceptor + H2O
-
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
4-methyl-3-nitrophenol + acceptor + H2O
-
437874
Pseudomonas putida
2-nitro-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
4-methylcatechol + acceptor + H2O
-
437874
Pseudomonas putida
3,4-dihydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
4-n-propylphenol + acceptor + H2O
-
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
additional information
no activity with 2-cresol
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
additional information
no activity with 4-methyl-2-nitrophenol, 2,4,5-trimethylphenol, 2-chloro-4,5-dimethylphenol, 2,4,6-trimethylphenol, 2,6-dibromo-4-methylphenol, L-tyrosine, 4-hydroxybenzaldehyde 3-cresol
437874
Pseudomonas putida
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.9.1
8.3
-
-
Pseudomonas putida
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.9.1
cytochrome c
enzyme contains covalently bound flavin and a type c cytochrome
Pseudomonas putida
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.9.1
cytochrome c
enzyme contains covalently bound flavin and a type c cytochrome
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.9.1
additional information
-
additional information
effect of pH-value and ionic strength on KM-value
Pseudomonas putida
1.17.9.1
0.0264
-
phenazine methosulfate
reaction with 4-n-propylphenol
Pseudomonas putida
1.17.9.1
0.366
-
phenazine methosulfate
reaction with 2-bromo-4-methylphenol
Pseudomonas putida
1.17.9.1
1.94
-
phenazine methosulfate
reaction with 4-ethylphenol
Pseudomonas putida
1.17.9.1
3.26
-
phenazine methosulfate
reaction with 4-hydroxybenzyl alcohol
Pseudomonas putida
1.17.9.1
5.23
-
phenazine methosulfate
reaction with 2,4-dimethylphenol
Pseudomonas putida
1.17.9.1
6.76
-
phenazine methosulfate
reaction with 4-cresol
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.17.9.1
(RS)-1-(4-hydroxyphenyl)ethanol + acceptor + H2O
-
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
1,4-hydroxyquinone + acceptor + H2O
-
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
2,4-xylenol + acceptor + H2O
i.e. 2,4-dimethylphenol
437874
Pseudomonas putida
3-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
2-bromo-4-methylphenol + acceptor + H2O
-
437874
Pseudomonas putida
3-bromo-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
2-methoxy-4-methylphenol + acceptor + H2O
-
437874
Pseudomonas putida
3-methoxy-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
3,4-dimethylphenol + acceptor + H2O
-
437874
Pseudomonas putida
2-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
3-fluoro-4-methylphenol + acceptor + H2O
-
437874
Pseudomonas putida
2-fluoro-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
4-cresol + acceptor + H2O
acceptor: phenazine methosulfate
437874
Pseudomonas putida
4-hydroxybenzaldehyde + reduced acceptor
-
437874
Pseudomonas putida
?
1.17.9.1
4-cresol + acceptor + H2O
no activity with 2,6-dichlorophenol-indophenol as acceptor
437874
Pseudomonas putida
4-hydroxybenzaldehyde + reduced acceptor
-
437874
Pseudomonas putida
?
1.17.9.1
4-cresol + acceptor + H2O
no activity with O2, K3Fe(CN)6 or methylene blue
437874
Pseudomonas putida
4-hydroxybenzaldehyde + reduced acceptor
-
437874
Pseudomonas putida
?
1.17.9.1
4-cresol + acceptor + H2O
the enzyme catalyzes both 4-cresol hydroxylation and further oxidation of the product, 4-hydroxybenzyl alcohol to 4-hydroxybenzaldehyde
437874
Pseudomonas putida
4-hydroxybenzaldehyde + reduced acceptor
-
437874
Pseudomonas putida
?
1.17.9.1
4-cresol + phenazine methosulfate + H2O
-
437874
Pseudomonas putida
4-hydroxybenzyl alcohol + reduced phenazine methosulfate
-
-
-
?
1.17.9.1
4-ethylphenol + acceptor + H2O
-
437874
Pseudomonas putida
1-(4'-hydroxyphenyl)ethanol + reduced acceptor
-
-
-
?
1.17.9.1
4-hydroxybenzyl alcohol + acceptor
-
437874
Pseudomonas putida
4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
4-isopropylphenol + acceptor + H2O
-
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
4-methyl-1-naphthol + acceptor + H2O
-
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
4-methyl-3-nitrophenol + acceptor + H2O
-
437874
Pseudomonas putida
2-nitro-4-hydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
4-methylcatechol + acceptor + H2O
-
437874
Pseudomonas putida
3,4-dihydroxybenzaldehyde + reduced acceptor
-
-
-
?
1.17.9.1
4-n-propylphenol + acceptor + H2O
-
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
additional information
no activity with 2-cresol
437874
Pseudomonas putida
?
-
-
-
?
1.17.9.1
additional information
no activity with 4-methyl-2-nitrophenol, 2,4,5-trimethylphenol, 2-chloro-4,5-dimethylphenol, 2,4,6-trimethylphenol, 2,6-dibromo-4-methylphenol, L-tyrosine, 4-hydroxybenzaldehyde 3-cresol
437874
Pseudomonas putida
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.9.1
8.3
-
-
Pseudomonas putida