Any feedback?
Literature summary extracted from
- NADP+-dependent aldehyde dehydrogenase from Acetobacter rancens CCM 1774: purification and properties (1988), J. Basic Microbiol., 28, 25-33.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.4 | ADP | - |
Acetobacter pasteurianus |  |
| 1.2.1.4 | EDTA | 0.5 mM, 50% inhibition | Acetobacter pasteurianus | |
| 1.2.1.4 | ethyl acetate | - |
Acetobacter pasteurianus | |
| 1.2.1.4 | Fe2+ | 1 mM ZnCl2, complete inhibition, irreversible | Acetobacter pasteurianus | |
| 1.2.1.4 | Hg2+ | 0.5 mM HgCl2, complete inhibition, irreversible | Acetobacter pasteurianus | |
| 1.2.1.4 | NADPH | product inhibition | Acetobacter pasteurianus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.4 | 0.019 | - |
NADP+ | in presence of propanal | Acetobacter pasteurianus | |
| 1.2.1.4 | 0.031 | - |
acetaldehyde | - |
Acetobacter pasteurianus | |
| 1.2.1.4 | 0.15 | - |
propanal | - |
Acetobacter pasteurianus | |
| 1.2.1.4 | 0.38 | - |
formaldehyde | - |
Acetobacter pasteurianus | |
| 1.2.1.4 | 0.98 | - |
Butanal | - |
Acetobacter pasteurianus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.2.1.4 | cytosol | - |
Acetobacter pasteurianus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.4 | 82000 | - |
gel filtration | Acetobacter pasteurianus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.4 | additional information | Acetobacter pasteurianus | the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids | ? | - |
? | |
| 1.2.1.4 | additional information | Acetobacter pasteurianus CCM 1774 | the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.4 | Acetobacter pasteurianus | - |
CCM 1774 | - |
| 1.2.1.4 | Acetobacter pasteurianus CCM 1774 | - |
CCM 1774 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.1.4 | - |
Acetobacter pasteurianus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.2.1.4 | an aldehyde + NADP+ + H2O = a carboxylate + NADPH + H+ | random kinetic mechanism | Acetobacter pasteurianus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.4 | additional information | - |
- |
Acetobacter pasteurianus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.2.1.4 | 4°C, phosphate buffer, pH 6.8-7.5, half-life: 5 months | Acetobacter pasteurianus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.4 | acetaldehyde + NADP+ + H2O | - |
Acetobacter pasteurianus | acetate + NADPH + H+ | - |
? | |
| 1.2.1.4 | acetaldehyde + NADP+ + H2O | - |
Acetobacter pasteurianus CCM 1774 | acetate + NADPH + H+ | - |
? | |
| 1.2.1.4 | butanal + NADP+ + H2O | - |
Acetobacter pasteurianus | butyrate + NADPH + H+ | - |
? | |
| 1.2.1.4 | butanal + NADP+ + H2O | - |
Acetobacter pasteurianus CCM 1774 | butyrate + NADPH + H+ | - |
? | |
| 1.2.1.4 | formaldehyde + NADP+ + H2O | low activity | Acetobacter pasteurianus | formate + NADPH + H+ | - |
? | |
| 1.2.1.4 | formaldehyde + NADP+ + H2O | low activity | Acetobacter pasteurianus CCM 1774 | formate + NADPH + H+ | - |
? | |
| 1.2.1.4 | additional information | the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids | Acetobacter pasteurianus | ? | - |
? | |
| 1.2.1.4 | additional information | the constitutive enzyme may function in the intracellular detoxification of short chain aldehydes by conversion to corresponding fatty acids | Acetobacter pasteurianus CCM 1774 | ? | - |
? | |
| 1.2.1.4 | propanal + NADP+ + H2O | - |
Acetobacter pasteurianus | propionate + NADPH + H+ | - |
? | |
| 1.2.1.4 | propanal + NADP+ + H2O | - |
Acetobacter pasteurianus CCM 1774 | propionate + NADPH + H+ | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.4 | 8.5 | - |
- |
Acetobacter pasteurianus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.4 | 5 | 9.2 | pH 5.0: 10% of maximal activity, pH 9.2: 15% of maximal activity | Acetobacter pasteurianus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.4 | 7.5 | 9.5 | 30 min, stable | Acetobacter pasteurianus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.4 | NADP+ | - |
Acetobacter pasteurianus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.4 | 0.043 | - |
NADPH | - |
Acetobacter pasteurianus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
