Literature summary extracted from
Dirmeier, R.; Keller, M.; Frey, G.; Huber, H.; Stetter, K.O.
Purification and properties of an extremely thermostable membrane-bound sulfur-reducing complex from the hyperthermophilic Pyrodictium abyssi (1998), Eur. J. Biochem., 252, 486-491.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.12.98.4 |
additional information |
no quinione, but enzyme complex contains cytochrome c and has two additional cytochromes, probably of b-type |
Pyrodictium abyssi |
|
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.12.98.4 |
2-(n-heptyl)-4-hydroxyquinoline N-oxide |
not affected by |
Pyrodictium abyssi |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.12.98.4 |
membrane |
- |
Pyrodictium abyssi |
16020 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.12.98.4 |
Cu |
1.2 mol per mol protein |
Pyrodictium abyssi |
|
1.12.98.4 |
Fe |
50-55 mol iron per mol protein |
Pyrodictium abyssi |
|
1.12.98.4 |
Ni |
1.6 mol per mol protein |
Pyrodictium abyssi |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.12.98.4 |
52000 |
- |
enzym complex, gel-filtration |
Pyrodictium abyssi |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.12.98.4 |
Pyrodictium abyssi |
- |
TAG 11, a sulfur-reducing complex |
- |
Oxidation Stability
EC Number |
Oxidation Stability |
Organism |
---|
1.12.98.4 |
the sulfur-reducing activity is irreversibly damaged by oxygen, anaerobic conditions: no loss of activity after one week, aerobic conditions: 80% loss of activity in 24 h |
Pyrodictium abyssi |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.12.98.4 |
enzyme complex |
Pyrodictium abyssi |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.12.98.4 |
H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 |
a sulfur-reducing complex including hydrogenase, a sulfur reductase and electron-transferring components described |
Pyrodictium abyssi |
|
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.12.98.4 |
7.56 |
- |
at 100°C |
Pyrodictium abyssi |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.12.98.4 |
room temperature, exposed to air, 24 h, 80% loss of activity |
Pyrodictium abyssi |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.12.98.4 |
sulfur + electron donor |
electron donor: molecular hydrogen |
Pyrodictium abyssi |
H2S + oxidized electron donor |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.12.98.4 |
More |
nine major polypeptides with molecular masses of 82000, 72000, 65000, 50000, 47000, 42000, 40000, 30000 and 24000 Da |
Pyrodictium abyssi |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
1.12.98.4 |
100 |
- |
enzyme extremely thermostable |
Pyrodictium abyssi |
1.12.98.4 |
100 |
- |
below 85°C about 10% loss of activity |
Pyrodictium abyssi |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.12.98.4 |
8.5 |
- |
- |
Pyrodictium abyssi |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
1.12.98.4 |
7.5 |
9.5 |
- |
Pyrodictium abyssi |