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Literature summary extracted from

  • Engbersen, A.M.T.; Franken, D.G.; Boers, G.H.J.; Stevens, E.M.B.; Trijbels, F.J.M.; Blom, H.J.
    Thermolabile 5,10-methylenetetrahydrofolate reductase as a cause of mild hyperhomocysteinemia (1995), Am. J. Hum. Genet., 56, 142-150.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.5.1.20 medicine deficiency and thermolability of enzyme as cause of mild homocysteinemia with premature vascular disease Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.20 S-adenosylmethionine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.20 0.019
-
 5-methyltetrahydrofolate
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor Homo sapiens homocysteine metabolism 5-methyltetrahydrofolate + oxidized acceptor 5-methyltetrahydrofolate is the main form of circulating folate ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor Homo sapiens physiological direction is the 5-methyltetrahydrofolate formation 5-methyltetrahydrofolate + oxidized acceptor 5-methyltetrahydrofolate is the main form of circulating folate ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor Homo sapiens remethylation of homocysteine to methionine 5-methyltetrahydrofolate + oxidized acceptor 5-methyltetrahydrofolate is the main form of circulating folate ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.20 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.20 lymphocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.20 additional information
-
 values of healthy and homocysteinemic humans before and after heat inactivation, specific activity of thermolabile variant of enzyme in homozygotes is 50% of normal enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor reverse reaction: menadione as electron acceptor Homo sapiens 5-methyltetrahydrofolate + oxidized acceptor
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor homocysteine metabolism Homo sapiens 5-methyltetrahydrofolate + oxidized acceptor 5-methyltetrahydrofolate is the main form of circulating folate ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor physiological direction is the 5-methyltetrahydrofolate formation Homo sapiens 5-methyltetrahydrofolate + oxidized acceptor 5-methyltetrahydrofolate is the main form of circulating folate ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor remethylation of homocysteine to methionine Homo sapiens 5-methyltetrahydrofolate + oxidized acceptor 5-methyltetrahydrofolate is the main form of circulating folate ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.20 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.20 additional information
-
 activity of enzyme from healthy and homocysteinemic humans after heat inactivation, the latter with a more thermolabile enzyme: residual activity in homozygotes is lower than 30% compared with 50% in control subjects Homo sapiens
1.5.1.20 40
-
 5 min, 17% loss of activity Homo sapiens
1.5.1.20 46
-
 5 min, 53% loss of activity Homo sapiens
1.5.1.20 49
-
 5 min, 70% loss of activity Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.20 FAD enzyme activity depends on the concentration of its cofactor FAD: maximum activity at 0.05 mM FAD Homo sapiens