BRENDA - Enzyme Database show

Methyl viologen- and ferredoxin-linked sulfite reductase (spinach)

Asada, K.; Tamura, G.; Bandurski, R.S.; Methods Enzymol. 17B, 528-539 (1971)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.8.7.1
stable in 0.01 M phosphate buffer, pH 7.7
Spinacia oleracea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.7.1
Spinacia oleracea
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.7.1
-
Spinacia oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.7.1
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.7.1
0.0242
-
-
Spinacia oleracea
Storage Stability
EC Number
Storage Stability
Organism
1.8.7.1
-20°C, stable for 3 months
Spinacia oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.7.1
sulfite + reduced ferredoxin
reduced methyl viologen as electron donor also
437699
Spinacia oleracea
hydrogen sulfide + oxidized ferredoxin + H2O
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.7.1
50
-
no activity loss after heating for 5 h at this temperature, activity loss after incubation at 60°C for 30 min
Spinacia oleracea
General Stability (protein specific)
EC Number
General Stability
Organism
1.8.7.1
stable in 0.01 M phosphate buffer, pH 7.7
Spinacia oleracea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.7.1
-
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.7.1
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.7.1
0.0242
-
-
Spinacia oleracea
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.8.7.1
-20°C, stable for 3 months
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.7.1
sulfite + reduced ferredoxin
reduced methyl viologen as electron donor also
437699
Spinacia oleracea
hydrogen sulfide + oxidized ferredoxin + H2O
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.7.1
50
-
no activity loss after heating for 5 h at this temperature, activity loss after incubation at 60°C for 30 min
Spinacia oleracea