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Literature summary extracted from

  • Rohrer, S.P.; Saz, H.J.; Nowak, T.
    Purification and characteriation of phosphoenolpyruvate carboxykinase from the parasitic helminth Ascaris suum (1986), J. Biol. Chem., 261, 13049-13055.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.32 ATP competitive against GTP Ascaris suum
4.1.1.32 ITP competitive against GTP, IDP and phosphoenolpyruvate Ascaris suum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.32 0.022
-
 GDP
-
 Ascaris suum
4.1.1.32 0.12
-
 phosphoenolpyruvate
-
 Ascaris suum
4.1.1.32 0.182
-
 IDP
-
 Ascaris suum
4.1.1.32 6.7
-
 CO2 with IDP as cosubstrate, Mn2+ does not affect the Km Ascaris suum
4.1.1.32 6.7
-
 CO2 CO2 in form of HCO3- Ascaris suum
4.1.1.32 7.1
-
 CO2 with GDP as cosubstrate, Mn2+ does not affect the Km Ascaris suum
4.1.1.32 7.1
-
 CO2 CO2 in form of HCO3- Ascaris suum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Co2+ activates Ascaris suum
4.1.1.32 Co2+ maximal activity at 4 mM Ascaris suum
4.1.1.32 Mg2+ no activation Ascaris suum
4.1.1.32 Mn2+ activates Ascaris suum
4.1.1.32 Mn2+ Km: 0.77 mM Ascaris suum
4.1.1.32 Mn2+ binds directly to the enzyme and binds to the nucleotide resulting in the metal-nucleotide complex Ascaris suum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.32 83000
-
 gel filtration Ascaris suum

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Ascaris suum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.32
-
 Ascaris suum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.32 2.93
-
-
 Ascaris suum

Storage Stability

EC Number Storage Stability Organism
4.1.1.32 -70°C, 0.05 M potassium phosphate, pH 6.8, 0.2 M sucrose, stable for at least 21 months Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GDP + phosphoenolpyruvate + CO2
-
 Ascaris suum GTP + oxaloacetate
-
 ?
4.1.1.32 IDP + phosphoenolpyruvate + CO2
-
 Ascaris suum ITP + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.32 monomer 1 * 75000-80000, SDS-PAGE Ascaris suum