BRENDA - Enzyme Database

The purification, characterization, and activation of phosphoenolpyruvate carboxykinase from chicken liver mitochondria

Hebda, C.A.; Nowak, T.; J. Biol. Chem. 257, 5503-5514 (1982)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
4.1.1.32
2-mercaptoethanol facilitates loss of activity
Gallus gallus
4.1.1.32
dithiothreitol stabilizes
Gallus gallus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.32
(Z)-Phosphoenol-3-bromopyruvate
linear competitive
Gallus gallus
4.1.1.32
2-oxoglutarate
above 5.0 mM, weak
Gallus gallus
4.1.1.32
alpha-(Dihydroxyphosphinylmethyl) acrylic acid
weak linear competitive
Gallus gallus
4.1.1.32
citrate
above 5.0 mM, weak
Gallus gallus
4.1.1.32
DL-isocitrate
above 5.0 mM, weak
Gallus gallus
4.1.1.32
malate
above 5.0 mM, weak
Gallus gallus
4.1.1.32
oxalate
above 5.0 mM, weak
Gallus gallus
4.1.1.32
succinate
above 5.0 mM, weak
Gallus gallus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.32
0.003
-
GDP
-
Gallus gallus
4.1.1.32
0.013
-
IDP
-
Gallus gallus
4.1.1.32
0.03
-
(Z)-3-fluorophosphoenolpyruvate
-
Gallus gallus
4.1.1.32
0.042
-
MnGDP-
-
Gallus gallus
4.1.1.32
0.067
-
phosphoenolpyruvate
-
Gallus gallus
4.1.1.32
0.13
-
ITP
-
Gallus gallus
4.1.1.32
0.197
-
MnIDP-
-
Gallus gallus
4.1.1.32
19
-
HCO3-
-
Gallus gallus
4.1.1.32
40
-
MnITP2-
-
Gallus gallus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.32
mitochondrion
-
Gallus gallus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.32
Mn2+
activates
Gallus gallus
4.1.1.32
additional information
no requirement for a monovalent cation
Gallus gallus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.32
70500
-
1 * 70500, SDS-PAGE under reducing conditions
Gallus gallus
4.1.1.32
75400
-
high speed equilibrium sedimentation
Gallus gallus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.32
Gallus gallus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.32
liver
-
Gallus gallus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.32
GDP + (Z)-3-fluorophosphoenolpyruvate + CO2
-
4350
Gallus gallus
?
-
-
-
-
4.1.1.32
GTP + oxaloacetate
-
4350
Gallus gallus
GDP + phosphoenolpyruvate + CO2
-
4350
Gallus gallus
-
4.1.1.32
ITP + oxaloacetate
-
4350
Gallus gallus
IDP + phosphoenolpyruvate + CO2
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.32
monomer
1 * 70500, SDS-PAGE under reducing conditions
Gallus gallus
General Stability (protein specific)
EC Number
General Stability
Organism
4.1.1.32
2-mercaptoethanol facilitates loss of activity
Gallus gallus
4.1.1.32
dithiothreitol stabilizes
Gallus gallus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.32
(Z)-Phosphoenol-3-bromopyruvate
linear competitive
Gallus gallus
4.1.1.32
2-oxoglutarate
above 5.0 mM, weak
Gallus gallus
4.1.1.32
alpha-(Dihydroxyphosphinylmethyl) acrylic acid
weak linear competitive
Gallus gallus
4.1.1.32
citrate
above 5.0 mM, weak
Gallus gallus
4.1.1.32
DL-isocitrate
above 5.0 mM, weak
Gallus gallus
4.1.1.32
malate
above 5.0 mM, weak
Gallus gallus
4.1.1.32
oxalate
above 5.0 mM, weak
Gallus gallus
4.1.1.32
succinate
above 5.0 mM, weak
Gallus gallus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.32
0.003
-
GDP
-
Gallus gallus
4.1.1.32
0.013
-
IDP
-
Gallus gallus
4.1.1.32
0.03
-
(Z)-3-fluorophosphoenolpyruvate
-
Gallus gallus
4.1.1.32
0.042
-
MnGDP-
-
Gallus gallus
4.1.1.32
0.067
-
phosphoenolpyruvate
-
Gallus gallus
4.1.1.32
0.13
-
ITP
-
Gallus gallus
4.1.1.32
0.197
-
MnIDP-
-
Gallus gallus
4.1.1.32
19
-
HCO3-
-
Gallus gallus
4.1.1.32
40
-
MnITP2-
-
Gallus gallus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.32
mitochondrion
-
Gallus gallus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.32
Mn2+
activates
Gallus gallus
4.1.1.32
additional information
no requirement for a monovalent cation
Gallus gallus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.32
70500
-
1 * 70500, SDS-PAGE under reducing conditions
Gallus gallus
4.1.1.32
75400
-
high speed equilibrium sedimentation
Gallus gallus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.32
liver
-
Gallus gallus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.32
GDP + (Z)-3-fluorophosphoenolpyruvate + CO2
-
4350
Gallus gallus
?
-
-
-
-
4.1.1.32
GTP + oxaloacetate
-
4350
Gallus gallus
GDP + phosphoenolpyruvate + CO2
-
4350
Gallus gallus
-
4.1.1.32
ITP + oxaloacetate
-
4350
Gallus gallus
IDP + phosphoenolpyruvate + CO2
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.32
monomer
1 * 70500, SDS-PAGE under reducing conditions
Gallus gallus