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Literature summary extracted from

  • Charles, A.M.; Sykora, Y.
    Purification and characterization of the phosphoenolpyruvate carboxylase from the facultative chemolithotroph Thiobacillus novellus (ATCC 8093) (1992), Antonie van Leeuwenhoek, 62, 155-165.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 acetoacetyl-CoA activates Starkeya novella
4.1.1.31 butyryl-CoA activates Starkeya novella
4.1.1.31 heptanoyl-CoA activates Starkeya novella
4.1.1.31 hexanoyl-CoA activates Starkeya novella
4.1.1.31 propionyl-CoA activates Starkeya novella
4.1.1.31 succinyl-CoA activates Starkeya novella
4.1.1.31 valeryl-CoA activates Starkeya novella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 additional information
-
additional information
-
Starkeya novella
4.1.1.31 0.11
-
HCO3-
-
Starkeya novella
4.1.1.31 0.64
-
phosphoenolpyruvate
-
Starkeya novella

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Cd2+ 28% of the activation with Mg2+ Starkeya novella
4.1.1.31 Co2+ 30% of the activation with Mg2+ Starkeya novella
4.1.1.31 Mg2+ divalent cation required, Mg2+ is the best activator Starkeya novella
4.1.1.31 Mn2+ 68% of the activation with Mg2+. At suboptimal concentrations of phosphoenolpyruvate and HCO3-, Mn2+ is more effective than Mg2+ Starkeya novella

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 95000
-
4 * 95000, SDS-PAGE Starkeya novella
4.1.1.31 360000
-
gel filtration Starkeya novella

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.31 Phosphoenolpyruvate + CO2 Starkeya novella function is probably anaplerotic ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Starkeya novella
-
strain ATCC 90893
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
Starkeya novella

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 51.09
-
-
Starkeya novella

Storage Stability

EC Number Storage Stability Organism
4.1.1.31 -20°C, 0.04 M Tris/HCl buffer, pH 7.8, 2.0 mM dithiothreitol, 2.0 mM MgCl2, 1.0 mM EDTA, 20% glycerol, 1.0 mM Pro, stable for at least 4 weeks Starkeya novella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
Starkeya novella phosphate + oxaloacetate
-
?
4.1.1.31 Phosphoenolpyruvate + CO2 function is probably anaplerotic Starkeya novella ?
-
?
4.1.1.31 phosphoenolpyruvate + HCO3-
-
Starkeya novella phosphate + oxaloacetate
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 tetramer 4 * 95000, SDS-PAGE Starkeya novella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 8
-
-
Starkeya novella

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.31 acetyl-CoA activates Starkeya novella