BRENDA - Enzyme Database

Purification and characterization of phosphoenolpyruvate carboxylase from a cyanobacterium

Owttrim, G.W.; Colman, B.; J. Bacteriol. 168, 207-212 (1986)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.31
3-Phosphoglyceric acid
-
Coccochloris peniocystis
4.1.1.31
pyruvate
activates
Coccochloris peniocystis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.31
2-oxoglutarate
-
Coccochloris peniocystis
4.1.1.31
alpha-Hydroxy-2-pyridylmethanesulfonate
-
Coccochloris peniocystis
4.1.1.31
ATP
-
Coccochloris peniocystis
4.1.1.31
citrate
-
Coccochloris peniocystis
4.1.1.31
isocitrate
-
Coccochloris peniocystis
4.1.1.31
Maleate
-
Coccochloris peniocystis
4.1.1.31
malonate
-
Coccochloris peniocystis
4.1.1.31
oxaloacetate
-
Coccochloris peniocystis
4.1.1.31
phosphate
-
Coccochloris peniocystis
4.1.1.31
Picolinic acid
-
Coccochloris peniocystis
4.1.1.31
quinolinic acid
-
Coccochloris peniocystis
4.1.1.31
SO42-
-
Coccochloris peniocystis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.31
0.6
-
phosphoenolpyruvate
-
Coccochloris peniocystis
4.1.1.31
0.8
-
HCO3-
-
Coccochloris peniocystis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.31
Co2+
60% of the activation with Mg2+
Coccochloris peniocystis
4.1.1.31
Mg2+
activates; Km: 0.27 mM
Coccochloris peniocystis
4.1.1.31
Mn2+
80% of the activation with Mg2+
Coccochloris peniocystis
4.1.1.31
NaCl
activation between 25 and 200 mM to a maximum of 14% a at 75 mM
Coccochloris peniocystis
4.1.1.31
Zn2+
60% of the activation with Mg2+
Coccochloris peniocystis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.31
560000
-
gel filtration
Coccochloris peniocystis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.31
Coccochloris peniocystis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.31
-
Coccochloris peniocystis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.31
8.87
-
-
Coccochloris peniocystis
Storage Stability
EC Number
Storage Stability
Organism
4.1.1.31
-80°C, concentrated, undesalted solution containing 33% v/v glycerol, stable for at least 6 weeks
Coccochloris peniocystis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.31
phosphoenolpyruvate + CO2
-
4307
Coccochloris peniocystis
phosphate + oxaloacetate
-
4307
Coccochloris peniocystis
-
4.1.1.31
phosphoenolpyruvate + HCO3-
-
4307
Coccochloris peniocystis
phosphate + oxaloacetate
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.31
40
-
-
Coccochloris peniocystis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.31
8
-
-
Coccochloris peniocystis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.31
NADPH
activates
Coccochloris peniocystis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.31
3-Phosphoglyceric acid
-
Coccochloris peniocystis
4.1.1.31
pyruvate
activates
Coccochloris peniocystis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.31
NADPH
activates
Coccochloris peniocystis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.31
2-oxoglutarate
-
Coccochloris peniocystis
4.1.1.31
alpha-Hydroxy-2-pyridylmethanesulfonate
-
Coccochloris peniocystis
4.1.1.31
ATP
-
Coccochloris peniocystis
4.1.1.31
citrate
-
Coccochloris peniocystis
4.1.1.31
isocitrate
-
Coccochloris peniocystis
4.1.1.31
Maleate
-
Coccochloris peniocystis
4.1.1.31
malonate
-
Coccochloris peniocystis
4.1.1.31
oxaloacetate
-
Coccochloris peniocystis
4.1.1.31
phosphate
-
Coccochloris peniocystis
4.1.1.31
Picolinic acid
-
Coccochloris peniocystis
4.1.1.31
quinolinic acid
-
Coccochloris peniocystis
4.1.1.31
SO42-
-
Coccochloris peniocystis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.31
0.6
-
phosphoenolpyruvate
-
Coccochloris peniocystis
4.1.1.31
0.8
-
HCO3-
-
Coccochloris peniocystis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.31
Co2+
60% of the activation with Mg2+
Coccochloris peniocystis
4.1.1.31
Mg2+
activates; Km: 0.27 mM
Coccochloris peniocystis
4.1.1.31
Mn2+
80% of the activation with Mg2+
Coccochloris peniocystis
4.1.1.31
NaCl
activation between 25 and 200 mM to a maximum of 14% a at 75 mM
Coccochloris peniocystis
4.1.1.31
Zn2+
60% of the activation with Mg2+
Coccochloris peniocystis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.31
560000
-
gel filtration
Coccochloris peniocystis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.31
-
Coccochloris peniocystis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.31
8.87
-
-
Coccochloris peniocystis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.1.31
-80°C, concentrated, undesalted solution containing 33% v/v glycerol, stable for at least 6 weeks
Coccochloris peniocystis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.31
phosphoenolpyruvate + CO2
-
4307
Coccochloris peniocystis
phosphate + oxaloacetate
-
4307
Coccochloris peniocystis
-
4.1.1.31
phosphoenolpyruvate + HCO3-
-
4307
Coccochloris peniocystis
phosphate + oxaloacetate
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.31
40
-
-
Coccochloris peniocystis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.31
8
-
-
Coccochloris peniocystis