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Literature summary extracted from

  • Chiu, A.O.S.; Suyama, Y.
    The absence of structural relationship between mitochondrial and cytoplasmic leucyl-tRNA synthetases from Tetrahymena pyriformis (1975), Arch. Biochem. Biophys., 171, 43-54.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 additional information
-
 additional information
-
 Tetrahymena pyriformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.4 cytoplasm
-
 Tetrahymena pyriformis 5737
-
6.1.1.4 mitochondrion
-
 Tetrahymena pyriformis 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.4 K+ 5.0 mM, mitochondrial enzyme, 2.5 mM, cytoplasmic enzyme Tetrahymena pyriformis
6.1.1.4 Mg2+ optimal concentration: 2.5 mM (mitochondrial enzyme), 10 mM (cytoplasmic enzyme) Tetrahymena pyriformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 90000 100000 gel filtration, sucrose density gradient centrifugation Tetrahymena pyriformis
6.1.1.4 100000
-
 1 * 100000, SDS-PAGE Tetrahymena pyriformis

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Tetrahymena pyriformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4
-
 Tetrahymena pyriformis

Storage Stability

EC Number Storage Stability Organism
6.1.1.4 -20°C, 50% glycerol, stable for at least 3 months Tetrahymena pyriformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Tetrahymena pyriformis AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 monomer 1 * 100000, SDS-PAGE Tetrahymena pyriformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.4 20
-
 mitochondrial enzyme Tetrahymena pyriformis
6.1.1.4 35
-
 cytoplasmic enzyme Tetrahymena pyriformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.1.1.4 5 40 5°C: about 35% of maximal activity, 40°C: about 45% of maximal activity, mitochondrial enzyme Tetrahymena pyriformis
6.1.1.4 20 40 20°C: about 30% of maximal activity, 40°C: about 85% of maximal activity, cytoplasmic enzyme Tetrahymena pyriformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.4 40
-
 2.5 min, 90% loss of activity of mitochondrial enzyme, 5-10 min, 90% loss of cytoplasmic enzyme Tetrahymena pyriformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 6 7 mitochondrial enzyme Tetrahymena pyriformis
6.1.1.4 7 8 cytoplasmic enzyme Tetrahymena pyriformis