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Literature summary extracted from

  • Floyd, E.E.; Jones, M.E.
    Isolation and characterization of the orotidine 5'-monophosphate decarboxylase domain of the multifunctional protein uridine 5'-monophosphate synthase (1985), J. Biol. Chem., 260, 9443-9451.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.1.23 glycerol, 50% v/v is an effective stabilizer of the orotidine-5'-phosphate decarboxylase domain at 4°C, -20°C or -70°C Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.10 51500
-
 x * 51500, SDS-PAGE, sedimentation studies reveal monomer or dimer, enzyme activity and orotidine 5-monophosphate decarboxylating activity on a single polypeptide Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Mus musculus
-
-
-
4.1.1.23 Mus musculus
-
 bifunctional UMP synthase contains activities of EC 2.4.2.10 and EC 4.1.1.23
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.23 preparation and isolation of the orotidine-5'-phosphate decarboxylase domain of UMP synthase Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.10 Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
4.1.1.23 -70°C, dilute buffer plus 2 mM dithiothreitol, purified UMP synthase activity retains orotidine-5'-phosphate decarboxylase activity for several months Mus musculus
4.1.1.23 0°C or -70°C, 5 mM potassium phosphate, pH 7.0, 2 mM dithiothreitol, rapid loss of orotidine-5'-phosphate decarboxylase activity of the orotidine-5'-phosphate decarboxylase domain of UMP synthase Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 additional information enzyme from mammals is a bifunctional polypeptide, it also catalyzes the reaction listed as EC 4.1.1.23 Mus musculus ?
-
 ?
4.1.1.23 Orotidine 5'-phosphate
-
 Mus musculus UMP + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.10 ? x * 51500, SDS-PAGE, sedimentation studies reveal monomer or dimer, enzyme activity and orotidine 5'-monophosphate decarboxylating activity on a single polypeptide Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.10 More enzyme from mammals is a bifunctional polypeptide, it also catalyzes the reaction listed as EC 4.1.1.23 Mus musculus