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Literature summary extracted from

  • Hyun, Y.L.; Davidson, V.L.
    Electron transfer reactions between aromatic amine dehydrogenase and azurin (1995), Biochemistry, 34, 12249-12254.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.9.2 0.044
-
 azurin in 0.25 M potassium phosphate buffer Alcaligenes faecalis
1.4.9.2 0.137
-
 azurin in 0.01 M potassium phosphate buffer Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.9.2 aromatic amines + acceptor + H2O Alcaligenes faecalis physiological acceptor: azurin, not amicyanin ? + reduced acceptor + NH3
-
 ?
1.4.9.2 aromatic amines + acceptor + H2O Alcaligenes faecalis IFO 14479 physiological acceptor: azurin, not amicyanin ? + reduced acceptor + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.9.2 Alcaligenes faecalis
-
-
-
1.4.9.2 Alcaligenes faecalis IFO 14479
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.9.2 ArCH2NH2 + H2O + 2 azurin
-
 Alcaligenes faecalis ArCHO + NH3 + 2 reduced azurin
-
 ?
1.4.9.2 ArCH2NH2 + H2O + 2 azurin
-
 Alcaligenes faecalis IFO 14479 ArCHO + NH3 + 2 reduced azurin
-
 ?
1.4.9.2 aromatic amines + acceptor + H2O physiological acceptor: azurin, not amicyanin Alcaligenes faecalis ? + reduced acceptor + NH3
-
 ?
1.4.9.2 aromatic amines + acceptor + H2O physiological acceptor: azurin, not amicyanin Alcaligenes faecalis IFO 14479 ? + reduced acceptor + NH3
-
 ?
1.4.9.2 tyramine + acceptor + H2O electron acceptor: azurin Alcaligenes faecalis 4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
 ?
1.4.9.2 tyramine + acceptor + H2O electron acceptor: azurin Alcaligenes faecalis IFO 14479 4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.9.2 64
-
 azurin in 0.01 M potassium phosphate buffer Alcaligenes faecalis
1.4.9.2 91
-
 azurin in 0.25 M potassium phosphate buffer Alcaligenes faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.9.2 tryptophan tryptophylquinone
-
 Alcaligenes faecalis