BRENDA - Enzyme Database

Biosynthesis of stizolobinic acid and stizolobic acid in higher plants

Saito, K.; Komamine, A.; Eur. J. Biochem. 82, 385-392 (1978)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.13.11.29
DTT stabilizes
Stizolobium hassjoo
1.13.11.30
DTT stabilizes
Stizolobium hassjoo
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.29
8-Hydroxyquinoline-5-sulfonate
90% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Al3+
95% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
alpha,alpha'-dipyridyl
-
Stizolobium hassjoo
1.13.11.29
Co2+
65% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Cu2+
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Fe2+
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Fe3+
90% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
glutathione
55% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
iodoacetate
55% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
KCN
-
Stizolobium hassjoo
1.13.11.29
Mg2+
65% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Mn2+
95% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Mo6+
65% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
N-ethylmaleimide
45% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Neocuproine
55% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
o-phenanthroline
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
p-chloromercuribenzoate
80% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Salicylaldoxime
60% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
8-Hydroxyquinoline-5-sulfonate
90% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Al3+
95% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
alpha,alpha'-dipyridyl
70% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Co2+
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Cu2+
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Fe2+
75% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Fe3+
95% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
glutathione
70% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
iodoacetate
55% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
KCN
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Mg2+
65% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Mn2+
95% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Mo6+
80% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
N-ethylmaleimide
35% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Neocuproine
50% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
o-phenanthroline
90% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
p-chloromercuribenzoate
70% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Salicylaldoxime
65% inhibition at 0.1 mM
Stizolobium hassjoo
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.29
0.00022
-
NADP+
-
Stizolobium hassjoo
1.13.11.29
1.39
-
3,4-dihydroxyphenylalanine
-
Stizolobium hassjoo
1.13.11.30
0.00026
-
NADP+
-
Stizolobium hassjoo
1.13.11.30
1.67
-
3,4-dihydroxyphenylalanine
-
Stizolobium hassjoo
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.29
Zn2+
activation
Stizolobium hassjoo
1.13.11.30
Zn2+
activation
Stizolobium hassjoo
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.29
45000
-
gel filtration
Stizolobium hassjoo
1.13.11.30
45000
-
gel filtration
Stizolobium hassjoo
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.13.11.29
3,4-dihydroxy-L-phenylalanine + O2
Stizolobium hassjoo
-
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
Stizolobium hassjoo
?
1.13.11.30
3,4-dihydroxy-L-phenylalanine + O2
Stizolobium hassjoo
-
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
Stizolobium hassjoo
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.13.11.29
Stizolobium hassjoo
-
-
-
1.13.11.30
Stizolobium hassjoo
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.13.11.29
-
Stizolobium hassjoo
1.13.11.30
-
Stizolobium hassjoo
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.29
seedling
etiolated
Stizolobium hassjoo
-
1.13.11.30
seedling
-
Stizolobium hassjoo
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.29
0.06
-
-
Stizolobium hassjoo
1.13.11.30
0.031
-
-
Stizolobium hassjoo
Storage Stability
EC Number
Storage Stability
Organism
1.13.11.29
-20°C, 0.1 mM DTT, stable for 3 months
Stizolobium hassjoo
1.13.11.29
30°C, half-life of 3-5 h
Stizolobium hassjoo
1.13.11.30
-20°C, 0.1 mM DTT, nitrogen atmosphere, stable for 3 months
Stizolobium hassjoo
1.13.11.30
30°C, purified enzyme, half life of 3-5 h
Stizolobium hassjoo
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.13.11.29
3,4-dihydroxy-L-phenylalanine + O2
-
396405
Stizolobium hassjoo
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
396405
Stizolobium hassjoo
?
1.13.11.29
3,4-dihydroxy-L-phenylalanine + O2
-
396405
Stizolobium hassjoo
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
undergoes ring closure and oxidation to stizolobate with NAD(P)+ as acceptor
396405
Stizolobium hassjoo
?
1.13.11.29
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
396405
Stizolobium hassjoo
stizolobate + NADPH + H+
-
-
-
r
1.13.11.30
3,4-dihydroxy-L-phenylalanine + O2
-
396405
Stizolobium hassjoo
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
396405
Stizolobium hassjoo
?
1.13.11.30
3,4-dihydroxy-L-phenylalanine + O2
-
396405
Stizolobium hassjoo
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
undergoes ring closure and oxidation to stizolobinate with NAD(P)+ as acceptor
396405
Stizolobium hassjoo
?
1.13.11.30
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
396405
Stizolobium hassjoo
stizolobinate + NADPH + H+
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.29
7.4
-
-
Stizolobium hassjoo
1.13.11.30
7.6
-
-
Stizolobium hassjoo
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.13.11.29
NAD+
required for reaction, less efficient than NADP+
Stizolobium hassjoo
1.13.11.29
NADP+
required for reaction
Stizolobium hassjoo
1.13.11.30
NAD+
required for reaction, less efficient than NADP+
Stizolobium hassjoo
1.13.11.30
NADP+
required for reaction
Stizolobium hassjoo
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.13.11.29
NAD+
required for reaction, less efficient than NADP+
Stizolobium hassjoo
1.13.11.29
NADP+
required for reaction
Stizolobium hassjoo
1.13.11.30
NAD+
required for reaction, less efficient than NADP+
Stizolobium hassjoo
1.13.11.30
NADP+
required for reaction
Stizolobium hassjoo
General Stability (protein specific)
EC Number
General Stability
Organism
1.13.11.29
DTT stabilizes
Stizolobium hassjoo
1.13.11.30
DTT stabilizes
Stizolobium hassjoo
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.29
8-Hydroxyquinoline-5-sulfonate
90% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Al3+
95% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
alpha,alpha'-dipyridyl
-
Stizolobium hassjoo
1.13.11.29
Co2+
65% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Cu2+
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Fe2+
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Fe3+
90% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
glutathione
55% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
iodoacetate
55% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
KCN
-
Stizolobium hassjoo
1.13.11.29
Mg2+
65% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Mn2+
95% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Mo6+
65% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
N-ethylmaleimide
45% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Neocuproine
55% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
o-phenanthroline
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
p-chloromercuribenzoate
80% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.29
Salicylaldoxime
60% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
8-Hydroxyquinoline-5-sulfonate
90% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Al3+
95% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
alpha,alpha'-dipyridyl
70% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Co2+
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Cu2+
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Fe2+
75% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Fe3+
95% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
glutathione
70% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
iodoacetate
55% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
KCN
85% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Mg2+
65% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Mn2+
95% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Mo6+
80% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
N-ethylmaleimide
35% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Neocuproine
50% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
o-phenanthroline
90% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
p-chloromercuribenzoate
70% inhibition at 0.1 mM
Stizolobium hassjoo
1.13.11.30
Salicylaldoxime
65% inhibition at 0.1 mM
Stizolobium hassjoo
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.29
0.00022
-
NADP+
-
Stizolobium hassjoo
1.13.11.29
1.39
-
3,4-dihydroxyphenylalanine
-
Stizolobium hassjoo
1.13.11.30
0.00026
-
NADP+
-
Stizolobium hassjoo
1.13.11.30
1.67
-
3,4-dihydroxyphenylalanine
-
Stizolobium hassjoo
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.29
Zn2+
activation
Stizolobium hassjoo
1.13.11.30
Zn2+
activation
Stizolobium hassjoo
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.29
45000
-
gel filtration
Stizolobium hassjoo
1.13.11.30
45000
-
gel filtration
Stizolobium hassjoo
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.13.11.29
3,4-dihydroxy-L-phenylalanine + O2
Stizolobium hassjoo
-
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
Stizolobium hassjoo
?
1.13.11.30
3,4-dihydroxy-L-phenylalanine + O2
Stizolobium hassjoo
-
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
Stizolobium hassjoo
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.29
-
Stizolobium hassjoo
1.13.11.30
-
Stizolobium hassjoo
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.29
seedling
etiolated
Stizolobium hassjoo
-
1.13.11.30
seedling
-
Stizolobium hassjoo
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.29
0.06
-
-
Stizolobium hassjoo
1.13.11.30
0.031
-
-
Stizolobium hassjoo
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.13.11.29
-20°C, 0.1 mM DTT, stable for 3 months
Stizolobium hassjoo
1.13.11.29
30°C, half-life of 3-5 h
Stizolobium hassjoo
1.13.11.30
-20°C, 0.1 mM DTT, nitrogen atmosphere, stable for 3 months
Stizolobium hassjoo
1.13.11.30
30°C, purified enzyme, half life of 3-5 h
Stizolobium hassjoo
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.13.11.29
3,4-dihydroxy-L-phenylalanine + O2
-
396405
Stizolobium hassjoo
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
396405
Stizolobium hassjoo
?
1.13.11.29
3,4-dihydroxy-L-phenylalanine + O2
-
396405
Stizolobium hassjoo
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
undergoes ring closure and oxidation to stizolobate with NAD(P)+ as acceptor
396405
Stizolobium hassjoo
?
1.13.11.29
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
396405
Stizolobium hassjoo
stizolobate + NADPH + H+
-
-
-
r
1.13.11.30
3,4-dihydroxy-L-phenylalanine + O2
-
396405
Stizolobium hassjoo
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
396405
Stizolobium hassjoo
?
1.13.11.30
3,4-dihydroxy-L-phenylalanine + O2
-
396405
Stizolobium hassjoo
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
undergoes ring closure and oxidation to stizolobinate with NAD(P)+ as acceptor
396405
Stizolobium hassjoo
?
1.13.11.30
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
396405
Stizolobium hassjoo
stizolobinate + NADPH + H+
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.29
7.4
-
-
Stizolobium hassjoo
1.13.11.30
7.6
-
-
Stizolobium hassjoo