Literature summary extracted from

Das, N.; Chakraborty, T.K.; Mukherjee, M.
Purification and characterization of a growth-regulating laccase from Pleurotus florida (2001), J. Basic Microbiol., 41, 261-267.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.10.3.2	azide	100 inhibition at 0.1 mM	Pleurotus floridanus
1.10.3.2	CN-	100% inhibition at 1 mM	Pleurotus floridanus
1.10.3.2	Fe2+	50% inhibition at 50 mM	Pleurotus floridanus
1.10.3.2	NaCl	80% inhibition at 50 mM	Pleurotus floridanus
1.10.3.2	sodium dodecylsulfate	50% inhibition at 1%	Pleurotus floridanus
1.10.3.2	Zn2+	50% inhibition at 50 mM	Pleurotus floridanus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.10.3.2	0.045	-	o-Dianisidine	isoenzyme L2	Pleurotus floridanus
1.10.3.2	2.8	-	guaiacol	isoenzyme L2	Pleurotus floridanus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.10.3.2	copper	2 gatom per mol	Pleurotus floridanus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.10.3.2	70000	-	1 * 70000, SDS-PAGE, isoenzyme L2	Pleurotus floridanus
1.10.3.2	73000	-	gel filtration, isoenzyme L2	Pleurotus floridanus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.10.3.2	Pleurotus floridanus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.10.3.2	isozyme L2	Pleurotus floridanus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.10.3.2	guaiacol + O2	-	Pleurotus floridanus	6-methoxycyclohexa-2,4-dienone + H2O	-	?
1.10.3.2	o-dianisidine	-	Pleurotus floridanus	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.10.3.2	monomer	1 * 70000, SDS-PAGE, isoenzyme L2	Pleurotus floridanus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.10.3.2	50	-	-	Pleurotus floridanus

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Release 2023.1 (January 2023)