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Literature summary extracted from

  • De Vrij, W.; Poolman, B.; Konings, W.N.; Azzi, A.
    Purification, enzymatic properties, and reconstitution of cytochrome-c oxidase from Bacillus subtilis (1986), Methods Enzymol., 126, 159-173.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.1.9 Tween 80
-
 Bacillus subtilis

General Stability

EC Number General Stability Organism
7.1.1.9 ultrafiltration causes inactivation Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.9 azide 0.08 mM, 50% inhibition Bacillus subtilis
7.1.1.9 CN- 0.001 mM, 50% inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.9 additional information
-
 additional information enzyme exhibits positive cooperativity at low ionic strength, increasing the KCl concentration to 25 mM causes loss of cooperativity Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.9 22000
-
 1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE Bacillus subtilis
7.1.1.9 37000
-
 1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE Bacillus subtilis
7.1.1.9 57000
-
 1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE Bacillus subtilis
7.1.1.9 290000 315000 enzyme associated with detergents Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.9 method 1: cytochrome c affinity chromatography, ion-exchange, method 2: ammonium sulfate, anion exchange, gel filtration Bacillus subtilis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
7.1.1.9 reconstitution Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 ferrocytochrome c + O2 horse ferrocytochrome c Bacillus subtilis ferricytochrome c + H2O
-
 ?
7.1.1.9 ferrocytochrome c + O2 artificial electron donor: ascorbate/hexaamine ruthenium Bacillus subtilis ferricytochrome c + H2O
-
 ?
7.1.1.9 ferrocytochrome c + O2 artificial electron donor: ascorbate/N,N,N',N'-tetramethyl-p-phenylenediamine Bacillus subtilis ferricytochrome c + H2O
-
 ?
7.1.1.9 ferrocytochrome c + O2 + H+
-
 Bacillus subtilis ferricytochrome c + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
7.1.1.9 trimer 1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.9 8 12 ferrocytochrome c in the presence of 0.1% dodecyl-beta-D-maltoside Bacillus subtilis