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Literature summary extracted from

  • Tachiki, T.; Nason, A.
    Preparation and proterties of apoenzyme of nitrate reductases from wild-type and nit-3 mutant of Neurospora crassa (1983), Biochim. Biophys. Acta, 744, 16-22.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.3 additional information enzyme can be reactivated by molybdenum and dithioerythritol Neurospora crassa

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.3 additional information nit-1 mutant: lacks all activities except FAD-dependent NADPH:cytochrome c reductase activity,nit-2 mutant: reduced FAD:- and reduced methyl viologen:nitrate reductase activities but lacks the other two activities Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.3 Molybdenum 1 mol per mol enzyme protein, activation of enzyme after urea treatment, little stimulation Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.3 132000
-
 2 * 97000: nit-3 enzyme, 2 * 132000, wild-type Neurospora crassa
1.7.1.3 204000
-
 nit-3 enzyme, gel filtration Neurospora crassa
1.7.1.3 272000
-
 wild-type, gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.3 nitrate + NADPH Neurospora crassa first step in nitrate assimilation nitrite + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Neurospora crassa
-
 wild-type STA4, nit-1 and nit-3 mutants
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.3 partially purification of wild-type and nit-3 mutant by streptomycin sulfate precipitation and ammonium sulfate fractionation, of nit-1 mutant by protamine sulfate fractionation, ammonium sulfate precipitation and gel filtration Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.3 mycelium
-
 Neurospora crassa
-

Storage Stability

EC Number Storage Stability Organism
1.7.1.3 5°C, urea-treated, 0.1 M potassium phosphate buffer, pH 7.3, 5 mM EDTA, 2 mM dithioerythritol, 0.5 mg/ml or more bovine serum albumin, stable for 18 h without loss of activity Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 nitrate + NADPH 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase Neurospora crassa nitrite + NADP+ + H2O
-
 ?
1.7.1.3 nitrate + NADPH first step in nitrate assimilation Neurospora crassa nitrite + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.3 dimer 2 * 97000: nit-3 enzyme, 2 * 132000, wild-type Neurospora crassa
1.7.1.3 More nit-1 mutant enzyme is the apoprotein of nitrate reductase Neurospora crassa
1.7.1.3 More aggregate of two different polypeptide chains: one responsible for transport of electrons fom NADPH to FAD or cytochrome c, nit-1 enzyme. The other transfers electrons from FAD via molybdenum to nitrate, nit-3 enzyme Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.3 cytochrome b557
-
 Neurospora crassa
1.7.1.3 heme 2 mol heme iron per mol enzyme protein Neurospora crassa