Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Padidam , M.; Venkateswarlu, K.; Johri, M.M.
    Ammonium represses NADPH-nitrate reductase in the moss Funaria hygrometrica (1991), Plant Sci., 75, 185-194.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.7.1.3 loss of activity with ammonium chloride or ammonium nitrate at 10 mM to growing cells, faster decay in washed and resuspended cells Funaria hygrometrica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.3 0.012 0.015 nitrate
-
 Funaria hygrometrica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.3 NH4+ represses the intracellular NO3- assimilation, which is required for the induction and maintenace of NADPH nitrate reductase, enzyme is regulated by NH4+ at the transcriptional level Funaria hygrometrica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.3 116000
-
 4 * 116000 Funaria hygrometrica
1.7.1.3 450000
-
 native gel electrophoresis Funaria hygrometrica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.3 nitrate + NADPH Funaria hygrometrica
-
 nitrite + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Funaria hygrometrica
-
 Hedw.
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.3 chloronema
-
 Funaria hygrometrica
-

Storage Stability

EC Number Storage Stability Organism
1.7.1.3 half life of about 2 h with ammonium sulfate or ammonium chloride as sole N-source Funaria hygrometrica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 nitrate + NADPH
-
 Funaria hygrometrica nitrite + NADP+ + H2O
-
 ?
1.7.1.3 nitrate + NADPH associated cytochrome c reductase activity Funaria hygrometrica nitrite + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.3 tetramer 4 * 116000 Funaria hygrometrica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.3 30
-
 assay at Funaria hygrometrica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.3 7.5
-
 assay at Funaria hygrometrica