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Literature summary extracted from

  • Chang, S.I.; Kang, M.K.; Choi, J.D.; Namgoong, S.K.
    Soluble overexpression in Escherichia coli, and purification and characterization of wild-type recombinant tobacco acetolactate synthase (1997), Biochem. Biophys. Res. Commun., 234, 549-553.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 cloned into the bacterial expression vector pGEX-2T and used to transform Escherichia coli strain pGEX-2T Nicotiana tabacum
2.2.1.6 wild-type recombinant enzyme fused with glutathione S-transferase Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 metsulfuron-methyl
-
 Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 12.1
-
 pyruvate wild-type recombinant enzyme Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 pyruvate Nicotiana tabacum first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Nicotiana tabacum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6
-
 Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Nicotiana tabacum 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile Nicotiana tabacum ?
-
 ?