Literature summary extracted from
Schloss, J.V.; VanDyk, D.E.; Vasta, J.F.; Kutny, R.M.
Purification and properties of Salmonella typhimurium acetolactate synthase isozyme II from Escherichia coli HB101/pDU9 (1985), Biochemistry, 24, 4952-4959.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.2.1.6 |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.2.1.6 |
Mg2+ |
required |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
2.2.1.6 |
Mg2+ |
Km: 0.022 mM |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.2.1.6 |
9800 |
- |
2 * 9800 + 2 * 59000, SDS-PAGE |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.2.1.6 |
59000 |
- |
2 * 9800 + 2 * 59000, SDS-PAGE |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.2.1.6 |
140000 |
- |
gel filtration |
Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.2.1.6 |
Salmonella enterica subsp. enterica serovar Typhimurium |
- |
isoenzyme II, recombinantly produced in Escherichia coli HB101/pDU9 |
- |
Oxidation Stability
EC Number |
Oxidation Stability |
Organism |
---|
2.2.1.6 |
oxygen-dependent inactivation of the enzyme complexed with thiamine diphosphate, FAD and magnesium acetolactate, no inactivation of the enzyme-FAD complex |
Salmonella enterica subsp. enterica serovar Typhimurium |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.2.1.6 |
isoenzyme II, recombinantly produced |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.2.1.6 |
25.3 |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.2.1.6 |
-80°C, concentrated enzyme solution is indefinitely stable |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.2.1.6 |
pyruvate |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
2-acetolactate + CO2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.2.1.6 |
tetramer |
2 * 9800 + 2 * 59000, SDS-PAGE |
Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.2.1.6 |
FAD |
contains bound FAD, 0.88 mol per mol of protomer of 69000 Da |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
2.2.1.6 |
thiamine diphosphate |
required as cofactor |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
2.2.1.6 |
thiamine diphosphate |
Km: 0.0015 mM |
Salmonella enterica subsp. enterica serovar Typhimurium |
|