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Literature summary extracted from
- Overproduction, purification and properties of 2,3-dihydroxyphenylpropionate 1,2-dioxygenase from Escherichia coli (1993), Biochim. Biophys. Acta, 1202, 258-264.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.13.11.16 | activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.16 | EDTA | - |
Escherichia coli |  |
| 1.13.11.16 | H2O2 | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.16 | 0.026 | - |
3-(2,3-dihydroxyphenyl)propionate | - |
Escherichia coli | |
| 1.13.11.16 | 0.09 | - |
3-methylcatechol | - |
Escherichia coli | |
| 1.13.11.16 | 0.72 | - |
catechol | - |
Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.16 | Fe2+ | weakly bound, 1 mol per mol tetramer, by addition of Fe2+ re-activated apoenzyme | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.16 | 36000 | - |
4 * 36000, SDS-PAGE | Escherichia coli |
| 1.13.11.16 | 134000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.16 | 3-(2,3-dihydroxyphenyl)propionate + O2 | Escherichia coli | - |
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.16 | Escherichia coli | P0ABR9 | gene mhpB | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.16 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.16 | 47.9 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.16 | 2,3-dihydroxyphenylpropionic acid + O2 | - |
Escherichia coli | 2-hydroxy-6-oxonona-dienoate | - |
? | |
| 1.13.11.16 | 3-(2,3-dihydroxyphenyl)propionate + O2 | - |
Escherichia coli | 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate | - |
? | |
| 1.13.11.16 | 3-methylcatechol + O2 | meta-ring cleavage | Escherichia coli | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| 1.13.11.16 | catechol + O2 | meta-ring-cleavage | Escherichia coli | 2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.16 | tetramer | 4 * 36000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.16 | 2 | - |
catechol | - |
Escherichia coli | |
| 1.13.11.16 | 3.6 | - |
3-methylcatechol | - |
Escherichia coli | |
| 1.13.11.16 | 29 | - |
3-(2,3-dihydroxyphenyl)propionate | - |
Escherichia coli | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.16 | 8 | 9.5 | - |
Escherichia coli |
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