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Literature summary extracted from

  • Roche, P.A.; Moorehead, T.J.; Hamilton, G.A.
    Purification and properties of hog liver 4-hydroxyphenylpyruvate dioxygenase (1982), Arch. Biochem. Biophys., 216, 62-73.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.27 1-butanol 5% v/v, 2.9fold activation Sus scrofa
1.13.11.27 1-propanol 10% v/v, 2.1fold activation Sus scrofa
1.13.11.27 acetone 10% v/v, 5.7fold activation Sus scrofa
1.13.11.27 acetonitrile 10% v/v, 3.6fold activation Sus scrofa
1.13.11.27 Cyclohexanol 5% v/v, 4.4fold activation Sus scrofa
1.13.11.27 dioxane 10% v/v, 2.8fold activation Sus scrofa
1.13.11.27 ethanol 10% v/v, 2fold activation Sus scrofa
1.13.11.27 ether 10% v/v, 2.6fold activation Sus scrofa
1.13.11.27 methanol 10% v/v, 1.6fold activation Sus scrofa
1.13.11.27 methyl-cellosolve 10% v/v, 2.2fold activation Sus scrofa
1.13.11.27 tetrahydrofuran 10% v/v, 6.7fold activation Sus scrofa

General Stability

EC Number General Stability Organism
1.13.11.27 stable to freezing and thawing Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.27 additional information
-
 additional information the Km-value is the same at 25°C and at 38°C Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.27 copper 0.4 atoms of Fe per 89000 Da enzyme. Removal of copper does not correlate with loss of activity Sus scrofa
1.13.11.27 Iron 0.9 atoms of Fe per 89000 Da enzyme. Removal of iron correlates with loss of activity Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.27 44000
-
 2 * 44000, nonidentical subunits, SDS-PAGE Sus scrofa
1.13.11.27 50000 54000 gel filtration Sus scrofa
1.13.11.27 89000
-
 equilibrium ultracentrifugation Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Sus scrofa
-
 hog
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.27 routinely stored in presence of O2 Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.27
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.27 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.27 39.6
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.13.11.27 -20°C, concentrated solution, 1 mg/ml, pH 6.0, stable for 6 months or more Sus scrofa
1.13.11.27 -20°C, on long-term storage the enzyme forms polymers, reversal by thiols Sus scrofa
1.13.11.27 4°C, dilute solutions, 0.1 mg/ml, 100 mM sodium acetate buffer, PH 6.0, stable for up to 2 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Sus scrofa homogentisate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.27 dimer 2 * 44000, nonidentical subunits, SDS-PAGE Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.27 additional information
-
 enzymatic rate increases approximately 5fold for every 10°C increase, Km-value is the same at 25°C and at 38°C Sus scrofa