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Literature summary extracted from
- Analysis of the kinetic and redox properties of the NADH peroxidase R303M mutant: correlation with the crystal structure (2000), Biochemistry, 39, 10353-10364.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.11.1.1 | - |
Enterococcus faecalis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.11.1.1 | hanging drop vapor diffusion method | Enterococcus faecalis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.11.1.1 | H10Q | lower Km for H2O2 | Enterococcus faecalis |
| 1.11.1.1 | R303M | significant conformational change, lower Km and kcat | Enterococcus faecalis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.1 | 0.00069 | - |
H2O2 | H10Q mutant | Enterococcus faecalis |  |
| 1.11.1.1 | 0.001 | - |
NADH | R303M mutant | Enterococcus faecalis | |
| 1.11.1.1 | 0.003 | - |
NADH | wild type | Enterococcus faecalis | |
| 1.11.1.1 | 0.0064 | - |
H2O2 | R303M mutant | Enterococcus faecalis | |
| 1.11.1.1 | 0.0068 | - |
NADH | H10Q mutant | Enterococcus faecalis | |
| 1.11.1.1 | 0.014 | - |
H2O2 | wild type | Enterococcus faecalis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.1 | Enterococcus faecalis | P37062 | wild type and mutants | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.1 | - |
Enterococcus faecalis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.1 | NADH + H2O2 | - |
Enterococcus faecalis | NAD+ + H2O | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.1 | 0.05 | - |
NADH | R303M mutant | Enterococcus faecalis | |
| 1.11.1.1 | 0.388 | - |
NADH | wild type | Enterococcus faecalis | |
| 1.11.1.1 | 0.395 | - |
NADH | H10Q mutant | Enterococcus faecalis | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.1 | FAD | - |
Enterococcus faecalis | |
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Release 2023.1 (January 2023)
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