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Literature summary extracted from

  • Clark, R.D.; Hind, G.
    Isolation of a five-polypeptide cytochrome b-f complex from spinach chloroplasts (1983), J. Biol. Chem., 258, 10348-10354.
    View publication on PubMed

General Stability

EC Number General Stability Organism
7.1.1.6 most stable in cholate buffer Spinacia oleracea
7.1.1.6 PMSF prevents proteolysis during purification Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.6 2-heptyl-4-hydroxyquinoline N-oxide inhibition at low concentrations, 0.001-0.002 mM Spinacia oleracea
7.1.1.6 2-Iodo-6-isopropyl-3-methyl-2',2,4'-trinitrodiphenyl ether
-
 Spinacia oleracea
7.1.1.6 5-(n-undecyl)-6-hydroxy-4,7-dioxobenzothiazole inhibition at low concentrations, 0.001-0.002 mM Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.6 0.009
-
 plastoquinol
-
 Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.6 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.6
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.6 leaf
-
 Spinacia oleracea
-

Storage Stability

EC Number Storage Stability Organism
7.1.1.6 0°C, 4 days months Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.6 plastoquinol + plastocyanin
-
 Spinacia oleracea plastoquinone + reduced plastocyanin
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.6 More complex consists of the following polypeptides: cytochrome f MW 33500 cytochrome b563 MW 22000, Rieske FeS protein MW 19000, subunit IV MW 16500, and a polypeptide with the molecular weight of 37000, SDS-PAGE Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.6 heme
-
 Spinacia oleracea