EC Number | General Stability | Organism |
---|---|---|
1.8.4.4 | ethylene glycol, 10%, stabilizes | Saccharomyces cerevisiae |
1.8.4.4 | glutathione stabilizes, stabilizing effect increased by cystine | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.4.4 | cell associated | - |
Saccharomyces cerevisiae | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.4 | cystine + GSH | Saccharomyces cerevisiae | - |
cysteine + GSSG | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.4 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.4 | - |
Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.4.4 | 61.6 | - |
beta-hydroxyethyldisulfide | Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
1.8.4.4 | -20°C, pH 5.8, 1 mM glutathione, several months | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.4 | cystine + GSH | - |
Saccharomyces cerevisiae | cysteine + GSSG | - |
? | |
1.8.4.4 | additional information | inactive with: insulin and other proteins | Saccharomyces cerevisiae | ? | - |
? | |
1.8.4.4 | additional information | at low substrate concentration the reaction rate is greater with L-cystine than with any of the other substances | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.4.4 | 54 | - |
crude state, quite labile to heat above 54°C | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.4.4 | 8.6 | - |
pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate | Saccharomyces cerevisiae |