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Literature summary extracted from

  • Nakai, C.; Horiike, K.; Kuramitsu, S.; Kagamiyama, H.; Nozaki, M.
    Three isoenzymes of catechol 1,2-dioxygenase (pyrocatechase) alphaalpha, alphabeta, and betabeta from Pseudomonas arvilla C-1 (1980), J. Biol. Chem., 265, 660-665.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.0019
-
 catechol isoenzymes 1 and 3 Pseudomonas putida
1.13.11.1 0.002
-
 catechol isoenzyme 2 Pseudomonas putida
1.13.11.1 0.096
-
 oxygen isoenzymes 1 and 2 Pseudomonas putida
1.13.11.1 0.098
-
 oxygen isoenzyme 3 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.1 Iron 1 gatom per mol enzyme protein Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 30000
-
 2 * 30000, isoenzyme 3, SDS-PAGE Pseudomonas putida
1.13.11.1 30000
-
 1 * 30000 (alpha) + 1 * 32000 (beta), isoenzyme 2, SDS-PAGE Pseudomonas putida
1.13.11.1 32000
-
 2 * 32000, isoenzyme 1, SDS-PAGE Pseudomonas putida
1.13.11.1 32000
-
 1 * 30000 (alpha) + 1 * 32000 (beta), isoenzyme 2, SDS-PAGE Pseudomonas putida
1.13.11.1 59000
-
 isoenzyme 3, gel filtration Pseudomonas putida
1.13.11.1 64000
-
 isoenzyme 2, gel filtration Pseudomonas putida
1.13.11.1 66900
-
 isoenzyme 1, gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Pseudomonas putida
-
 C-1
-
1.13.11.1 Pseudomonas putida C-1
-
 C-1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2 poor substrate Pseudomonas putida (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 3-methylcatechol + O2 poor substrate Pseudomonas putida C-1 (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Pseudomonas putida (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Pseudomonas putida C-1 (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
 ?
1.13.11.1 catechol + O2
-
 Pseudomonas putida cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Pseudomonas putida C-1 cis,cis-muconate
-
 ?
1.13.11.1 additional information both intradiol and extradiol cleavage activities toward 3-substituted catechols Pseudomonas putida ?
-
 ?
1.13.11.1 additional information both intradiol and extradiol cleavage activities toward 3-substituted catechols Pseudomonas putida C-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 heterodimer 1 * 30000 (alpha) + 1 * 32000 (beta), isoenzyme 2, SDS-PAGE Pseudomonas putida
1.13.11.1 homodimer 2 * 32000, isoenzyme 1, SDS-PAGE Pseudomonas putida
1.13.11.1 homodimer 2 * 30000, isoenzyme 3, SDS-PAGE Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.1 45
-
 catechol isoenzyme 3 Pseudomonas putida
1.13.11.1 49
-
 catechol isoenzyme 2 Pseudomonas putida
1.13.11.1 54
-
 catechol isoenzyme 1 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7.5
-
 isoenzymes 1,2 and 3 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.1 7.5 9 isoenzymes 1,2 and 3 Pseudomonas putida