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Literature summary extracted from

  • Serrato, A.J.; Perez-Ruiz, J.M.; Cejudo, F.J.
    Cloning of thioredoxin h reductase and characterization of the thioredoxin reductase-thioredoxin h system from wheat (2002), Biochem. J., 367, 491-497.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.9 cysteine
-
 Triticum aestivum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expression in Escherichia coli as a His-tagged protein Triticum aestivum
1.8.1.9 expression of wild-type as His-tagged protein and mutant C53S in Escherichia coli, DNA sequence analysis Triticum aestivum

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.9 C53S site-directed mutagenesis, no activity since the redox cycle system is abolished Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.0004
-
 NADPH
-
 Triticum aestivum
1.8.1.9 0.0004
-
 NADPH wild-type Triticum aestivum
1.8.1.9 0.00076
-
 thioredoxin assay with DTNB instead of NADPH Triticum aestivum
1.8.1.9 0.0076
-
 thioredoxin disulfide h
-
 Triticum aestivum
1.8.1.9 0.036
-
 thioredoxin wild-type, thioredoxin from E. coli Triticum aestivum
1.8.1.9 0.6
-
 NADH
-
 Triticum aestivum
1.8.1.9 0.6
-
 NADH wild-type Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 35000
-
 x * 35000, SDS-PAGE Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Triticum aestivum Q8VX47
-
-
1.8.1.9 Triticum aestivum Q8VX47 cv. Chineses Spring
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 recombinant, wild-type and mutant C53S Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 leaf
-
 Triticum aestivum
-
1.8.1.9 leaf from mature plants, high accumulation Triticum aestivum
-
1.8.1.9 root from 2-4-day-old seedlings, high accumulation Triticum aestivum
-
1.8.1.9 seed aleurone layer, scutellum, starchy endosperm Triticum aestivum
-
1.8.1.9 seed low accumulation Triticum aestivum
-
1.8.1.9 seedling
-
 Triticum aestivum
-
1.8.1.9 seedling root and shoot Triticum aestivum
-
1.8.1.9 shoot from 2-4-day-old seedlings, high accumulation Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 additional information redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors Triticum aestivum ?
-
 ?
1.8.1.9 thioredoxin + NADP+ coupled assay with DTNB Triticum aestivum thioredoxin disulfide + NADPH
-
 ?
1.8.1.9 thioredoxin + NADP+ coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin Triticum aestivum thioredoxin disulfide + NADPH
-
 ?
1.8.1.9 thioredoxin + NADP+ in presence of NADPH, coupled assay Triticum aestivum thioredoxin disulfide + NADPH + H+
-
 ?
1.8.1.9 thioredoxin + NADP+ i.e. DTNB Triticum aestivum thioredoxin disulfide + NADPH + H+
-
 ?
1.8.1.9 thioredoxin disulfide h + NADH
-
 Triticum aestivum thioredoxin + NAD+
-
 ?
1.8.1.9 thioredoxin disulfide h + NADPH
-
 Triticum aestivum thioredoxin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 ? x * 35000, SDS-PAGE Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 NTR
-
 Triticum aestivum
1.8.1.9 thioredoxin h reductase
-
 Triticum aestivum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Triticum aestivum
1.8.1.9 NADPH specific for Triticum aestivum