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Literature summary extracted from
- Enzymatic synthesis of desoxyribonucleotides. V. Purification and properties of thioredoxin reductase from Escherichia coli B (1964), J. Biol. Chem., 239, 3445-3452.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | KCN | - |
Escherichia coli |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | arsenite | - |
Escherichia coli | |
| 1.8.1.9 | CdCl2 | - |
Escherichia coli | |
| 1.8.1.9 | p-mercuribenzoate | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 68000 | - |
sedimentation equilibrium, ultracentrifugation | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | additional information | - |
- |
Escherichia coli |
| 1.8.1.9 | 58 | - |
purified enzyme | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | additional information | slowly reduces other proteins, e.g. insulin, lipoate and ribonuclease | Escherichia coli | ? | - |
? | |
| 1.8.1.9 | thioredoxin + NADP+ | - |
Escherichia coli | thioredoxin disulfide + NADPH | - |
ir | |
| 1.8.1.9 | thioredoxin + NADP+ | - |
Escherichia coli | thioredoxin disulfide + NADPH + H+ | - |
ir | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 65 | - |
7 min, active | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 7 | - |
assay at | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | FAD | - |
Escherichia coli | |
| 1.8.1.9 | NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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