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Literature summary extracted from

  • Schnurr, K.; Borchert, A.; Gerth, C.; Anton, M.; Kuhn, H.
    Bacterial and nonbacterial expression of wild-type and mutant human phospholipid hydroperoxide glutathione peroxidase and purification of the mutant enzyme in the milligram scale (2000), Protein Expr. Purif., 19, 403-410.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.12 expression of wild-type enzyme and SeC46C mutant in baculovirus/insect cell system and expression of mutant SeC46C as His-tagged fusion protein in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.12 SeC46C expression of mutant in Escherichia coli as His-tagged fusion protein and in baculovirus/insect cell system, site directed mutagenesis to avoid recognition problems of selenocysteine encoded by TGA stop codon in the expression systems, mutant has reduced specific acitvity Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.12 Se enzyme contains a selenocysteine residue at the active site Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.12 glutathione + lipid hydroperoxide Homo sapiens
-
 glutathione disulfide + lipid + H2O
-
 ?
1.11.1.12 additional information Homo sapiens phospholipid hydroperoxide glutathione peroxidase and 15-lipoxygenase are counterparts in the metabolism of hydroperoxy lipids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.12 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.12 large-scale, wild-type and mutant SeC46C from baculovirus/insect cell expression system and mutant SeC46C from expression in Escherichia coli as His-tagged fusion protein Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.12 additional information
-
 mutant compared to wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.12 glutathione + dilinoleoyl phosphatidylcholine hydroperoxide
-
 Homo sapiens ?
-
 ?
1.11.1.12 glutathione + lipid hydroperoxide
-
 Homo sapiens glutathione disulfide + lipid + H2O
-
 ?
1.11.1.12 additional information phospholipid hydroperoxide glutathione peroxidase and 15-lipoxygenase are counterparts in the metabolism of hydroperoxy lipids Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.12 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.12 additional information
-
 additional information mutant compared to wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.12 7.4
-
 assay at Homo sapiens