Any feedback?
Literature summary extracted from
- Site-directed mutagenesis of the redox-active cysteines of Trypanosoma cruzi trypanothione reductase (1995), Eur. J. Biochem., 228, 745-752.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.12 | additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma cruzi |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.12 | overexpression of wild-type and mutants C53S, C53A, C58S in Escherichia coli | Trypanosoma cruzi |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.1.12 | C53A | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| 1.8.1.12 | C53S | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| 1.8.1.12 | C58S | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.12 | 0.004 | 0.015 | thio-NADP+ | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi |  |
| 1.8.1.12 | 0.007 | 0.014 | NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
| 1.8.1.12 | 0.017 | - |
NADPH | recombinant wild-type | Trypanosoma cruzi | |
| 1.8.1.12 | 0.024 | - |
trypanothione disulfide | recombinant wild-type | Trypanosoma cruzi | |
| 1.8.1.12 | 0.046 | - |
glutathionylspermidine disulfide | recombinant wild-type | Trypanosoma cruzi | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.12 | Trypanosoma cruzi | - |
Silvio strain | - |
| 1.8.1.12 | Trypanosoma cruzi Silvio | - |
Silvio strain | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.12 | recombinant wild-type and mutants C53S, C53A, C58S from Escherichia coli | Trypanosoma cruzi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.12 | 0.18 | - |
recombinant mutant C58S: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| 1.8.1.12 | 0.21 | - |
recombinant mutant C53A: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| 1.8.1.12 | 0.44 | - |
recombinant mutant C53S: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| 1.8.1.12 | 6.49 | - |
recombinant wild-type: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| 1.8.1.12 | 143.2 | - |
purified recombinant enzyme | Trypanosoma cruzi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.12 | glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi | 2-glutathionylspermidine + NADP+ | - |
? | |
| 1.8.1.12 | glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi Silvio | 2-glutathionylspermidine + NADP+ | - |
? | |
| 1.8.1.12 | additional information | wild-type enzyme shows transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi | ? | - |
? | |
| 1.8.1.12 | additional information | recombinant mutants C53S, C53A, C58S: no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi | ? | - |
? | |
| 1.8.1.12 | additional information | wild-type enzyme shows transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi Silvio | ? | - |
? | |
| 1.8.1.12 | additional information | recombinant mutants C53S, C53A, C58S: no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi Silvio | ? | - |
? | |
| 1.8.1.12 | NADPH + H+ + thio-NADP+ | - |
Trypanosoma cruzi | NADP+ + thio-NADPH + H+ | - |
r | |
| 1.8.1.12 | NADPH + H+ + thio-NADP+ | - |
Trypanosoma cruzi Silvio | NADP+ + thio-NADPH + H+ | - |
r | |
| 1.8.1.12 | trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
? | |
| 1.8.1.12 | trypanothione disulfide + NADPH | - |
Trypanosoma cruzi Silvio | trypanothione + NADP+ | - |
? | |
| 1.8.1.12 | trypanothione disulfide + thio-NADPH + H+ | - |
Trypanosoma cruzi | trypanothione + thio-NADP+ | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.12 | TR | - |
Trypanosoma cruzi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.12 | 25 | - |
assay at | Trypanosoma cruzi |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.12 | 0.19 | 0.47 | thio-NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
| 1.8.1.12 | 0.21 | 0.51 | NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
| 1.8.1.12 | 6.33 | - |
NADPH | recombinant wild-type | Trypanosoma cruzi | |
| 1.8.1.12 | 7.02 | - |
thio-NADPH | recombinant wild-type | Trypanosoma cruzi | |
| 1.8.1.12 | 85.4 | - |
glutathionylspermidine disulfide | recombinant wild-type | Trypanosoma cruzi | |
| 1.8.1.12 | 108 | - |
trypanothione disulfide | recombinant wild-type | Trypanosoma cruzi | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.12 | beta-thio-NADPH | - |
Trypanosoma cruzi | |
| 1.8.1.12 | FAD | - |
Trypanosoma cruzi | |
| 1.8.1.12 | NADPH | - |
Trypanosoma cruzi | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
