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Literature summary extracted from
- Purification and characterization of glutathione reductase from Chlamydomonas reinhardtii (1993), J. Gen. Microbiol., 139, 2233-2238.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | Co2+ | complete inhibition at 1 mM | Chlamydomonas reinhardtii |  |
| 1.8.1.7 | Cu2+ | complete inhibition at 1 mM | Chlamydomonas reinhardtii | |
| 1.8.1.7 | Hg2+ | complete inhibition at 1 mM | Chlamydomonas reinhardtii | |
| 1.8.1.7 | Mn2+ | 40% inhibition | Chlamydomonas reinhardtii | |
| 1.8.1.7 | N-ethylmaleimide | - |
Chlamydomonas reinhardtii | |
| 1.8.1.7 | Ni2+ | 80% inhibition | Chlamydomonas reinhardtii | |
| 1.8.1.7 | p-chloromercuribenzoate | - |
Chlamydomonas reinhardtii | |
| 1.8.1.7 | Zn2+ | 75% inhibition | Chlamydomonas reinhardtii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.7 | 0.0106 | - |
NADPH | - |
Chlamydomonas reinhardtii | |
| 1.8.1.7 | 0.054 | - |
GSSG | - |
Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 54000 | 56000 | gel filtration | Chlamydomonas reinhardtii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | - |
Chlamydomonas reinhardtii |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.1.7 | 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | branching mechanism | Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 373.3 | - |
purified enzyme | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | GSSG + NADPH | highly specific for GSSG | Chlamydomonas reinhardtii | glutathione + NADP+ | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | monomer | 1 * 54000-56000, SDS-PAGE | Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 49 | - |
- |
Chlamydomonas reinhardtii |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 31 | 54 | 49% activity at 31°C and 68% activity at 54°C | Chlamydomonas reinhardtii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 45 | - |
stable | Chlamydomonas reinhardtii |
| 1.8.1.7 | 55 | - |
complete loss of activity | Chlamydomonas reinhardtii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 8.2 | - |
- |
Chlamydomonas reinhardtii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 6.8 | 8.4 | - |
Chlamydomonas reinhardtii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | FAD | FAD enzyme | Chlamydomonas reinhardtii | |
| 1.8.1.7 | additional information | no activity with FMN | Chlamydomonas reinhardtii | |
| 1.8.1.7 | NADPH | absolute specificity for NADPH | Chlamydomonas reinhardtii | |
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Release 2023.1 (January 2023)
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