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Literature summary extracted from

  • Mata, A.M.; Pinto, M.C.
    Purification by affinity chromatography of glutathione reductase (EC 1.6.4.2) from Escherichia coli and characterization of such enzyme (1984), Z. Naturforsch. C, 39, 908-915.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.1.7 dithiothreitol stabilizes Escherichia coli
1.8.1.7 FAD, stabilizes Escherichia coli
1.8.1.7 glycerol stabilizes Escherichia coli
1.8.1.7 NADP+ stabilizes Escherichia coli
1.8.1.7 pure enzyme heated in presence of bovine serum albumin and 0.020 mM NADPH: loss of activity beyond 30°C, significant reduction even at 0°C Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 additional information
-
 additional information
-
 Escherichia coli
1.8.1.7 0.016
-
 NADPH
-
 Escherichia coli
1.8.1.7 0.066
-
 GSSG
-
 Escherichia coli
1.8.1.7 0.377
-
 NADH
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 55000
-
 2 * 55000 Escherichia coli
1.8.1.7 109000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 GSSG + NADPH Escherichia coli
-
 glutathione + NADP+
-
 r
1.8.1.7 GSSG + NADPH Escherichia coli S33
-
 glutathione + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Escherichia coli
-
-
-
1.8.1.7 Escherichia coli S33
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 361
-
 purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.8.1.7 -18°C, in the dark, 10% glycerol, 5 mM NADP+, for at least 1 year Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADH + H+
-
 Escherichia coli glutathione + NAD+
-
 ?
1.8.1.7 glutathione disulfide + NADH + H+
-
 Escherichia coli S33 glutathione + NAD+
-
 ?
1.8.1.7 GSSG + NADPH
-
 Escherichia coli glutathione + NADP+
-
 r
1.8.1.7 GSSG + NADPH
-
 Escherichia coli S33 glutathione + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.1.7 dimer 2 * 55000 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 30
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.7 70
-
 highly stable, significant loss of activity only after 10 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 4.5
-
 NADH + GSSG Escherichia coli
1.8.1.7 7.5
-
 NADPH + GSSG Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.1.7 7.5 9.5
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.1.7 7.5
-
 unstable below Escherichia coli
1.8.1.7 7.5 9.5 4°C, stable Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 flavin flavoprotein Escherichia coli
1.8.1.7 NADPH
-
 Escherichia coli