BRENDA - Enzyme Database

Purification and properties of a new glutathione-dependent thiol:disulphide oxidoreductase from rat liver

Battelli, M.G.; Lorenzoni, E.; Biochem. J. 207, 133-138 (1982) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.4.7
additional information
insensitive against 1 mM CuSO4 and 1 mM N-ethylmaleimide at 37°C for 30 min
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.4.7
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.4.7
40000
-
1 * 40000, SDS-PAGE
Rattus norvegicus
1.8.4.7
43000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.17.1.4
xanthine + NAD+ + H2O
Rattus sp.
conversion of xanthine dehydrogenase to the oxidase type by thiol-disulfide oxidoreductase, thiol reagents or oxidized glutathione
urate + NADH
-
Rattus sp.
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.8.4.7
Rattus norvegicus
-
newborn and adults
-
1.17.1.4
Rattus sp.
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.8.4.7
ion-exchange, gel filtration
Rattus norvegicus
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
1.8.4.7
[xanthine dehydrogenase] + glutathione disulfide = [xanthine oxidase] + 2 glutathione
thioltransferase, GSH:insulin transhydrogenase and ricin-disulphide-bond reductase activity
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.4.7
brain
-
Rattus norvegicus
-
1.8.4.7
heart
-
Rattus norvegicus
-
1.8.4.7
kidney
-
Rattus norvegicus
-
1.8.4.7
liver
highest amount of activity
Rattus norvegicus
-
1.8.4.7
lung
-
Rattus norvegicus
-
1.8.4.7
skeletal muscle
-
Rattus norvegicus
-
1.8.4.7
skin
-
Rattus norvegicus
-
1.8.4.7
spleen
-
Rattus norvegicus
-
1.17.1.4
liver
-
Rattus sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.4.7
additional information
-
372 U/mg, other definition of units
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.8.4.7
additional information
other disulfide compounds are either inactive or far less active than oxidized glutathione, reduction of disulfide bonds in ricin, thioltransferase activity, GSH:insulin transhydrogenase activity
394699
Rattus norvegicus
?
-
-
-
?
1.8.4.7
[xanthine dehydrogenase] + oxidized glutathione
conversion into oxidase by treatment with proteolytic enzymes, storage at -20°C/-25°C or preincubation with either thiol reagents or whole liver homogenate. Can act in the opposite direction in the reduction of the disulfide bond of insulin and ricin
394699
Rattus norvegicus
[xanthine oxidase] + glutathione
-
394699
Rattus norvegicus
r
1.17.1.4
xanthine + NAD+ + H2O
conversion of xanthine dehydrogenase to the oxidase type by thiol-disulfide oxidoreductase, thiol reagents or oxidized glutathione
394699
Rattus sp.
urate + NADH
-
394699
Rattus sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.4.7
monomer
1 * 40000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.4.7
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.4.7
8.1
-
assay at
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
NAD+
-
Rattus sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.4
NAD+
-
Rattus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.4.7
additional information
insensitive against 1 mM CuSO4 and 1 mM N-ethylmaleimide at 37°C for 30 min
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.4.7
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.4.7
40000
-
1 * 40000, SDS-PAGE
Rattus norvegicus
1.8.4.7
43000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.17.1.4
xanthine + NAD+ + H2O
Rattus sp.
conversion of xanthine dehydrogenase to the oxidase type by thiol-disulfide oxidoreductase, thiol reagents or oxidized glutathione
urate + NADH
-
Rattus sp.
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.4.7
ion-exchange, gel filtration
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.4.7
brain
-
Rattus norvegicus
-
1.8.4.7
heart
-
Rattus norvegicus
-
1.8.4.7
kidney
-
Rattus norvegicus
-
1.8.4.7
liver
highest amount of activity
Rattus norvegicus
-
1.8.4.7
lung
-
Rattus norvegicus
-
1.8.4.7
skeletal muscle
-
Rattus norvegicus
-
1.8.4.7
skin
-
Rattus norvegicus
-
1.8.4.7
spleen
-
Rattus norvegicus
-
1.17.1.4
liver
-
Rattus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.4.7
additional information
-
372 U/mg, other definition of units
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.8.4.7
additional information
other disulfide compounds are either inactive or far less active than oxidized glutathione, reduction of disulfide bonds in ricin, thioltransferase activity, GSH:insulin transhydrogenase activity
394699
Rattus norvegicus
?
-
-
-
?
1.8.4.7
[xanthine dehydrogenase] + oxidized glutathione
conversion into oxidase by treatment with proteolytic enzymes, storage at -20°C/-25°C or preincubation with either thiol reagents or whole liver homogenate. Can act in the opposite direction in the reduction of the disulfide bond of insulin and ricin
394699
Rattus norvegicus
[xanthine oxidase] + glutathione
-
394699
Rattus norvegicus
r
1.17.1.4
xanthine + NAD+ + H2O
conversion of xanthine dehydrogenase to the oxidase type by thiol-disulfide oxidoreductase, thiol reagents or oxidized glutathione
394699
Rattus sp.
urate + NADH
-
394699
Rattus sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.4.7
monomer
1 * 40000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.4.7
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.4.7
8.1
-
assay at
Rattus norvegicus