Literature summary extracted from

Schwoerer, B.; Fernandez, V.M.; Zirngibl, C.; Thauer, R.K.
H2-Forming N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum (1993), Eur. J. Biochem., 212, 255-261.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.12.98.2	0.04	-	N5,N10-methylenetetrahydromethanopterin	-	Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.12.98.2	43000	-	x * 43000, SDS-PAGE	Methanothermobacter thermautotrophicus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.12.98.2	Methanothermobacter thermautotrophicus	-	-	-
1.12.98.2	Methanothermobacter thermautotrophicus Marburg / DSM 2133	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.12.98.2	N5,N10-methylenetetrahydromethanopterin + H+	r	Methanothermobacter thermautotrophicus	N5,N10-methenyltetrahydromethanopterin + H2	-	?
1.12.98.2	N5,N10-methylenetetrahydromethanopterin + H+	r	Methanothermobacter thermautotrophicus Marburg / DSM 2133	N5,N10-methenyltetrahydromethanopterin + H2	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.12.98.2	monomer	x * 43000, SDS-PAGE	Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.12.98.2	60	-	-	Methanothermobacter thermautotrophicus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.12.98.2	6.5	-	-	Methanothermobacter thermautotrophicus

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Release 2023.1 (January 2023)