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Literature summary extracted from

  • Wu, K.; Knox, R.; Sun, X.Z.; Joseph, P.; Jaiswal, A.K.; Zhang, D.; Deng, P.S.K.; Chen, S.
    Catalytic properties of NAD(P)H:quinone oxidoreductase-2 (NQO2), a dihydronicotinamide riboside dependent oxidoreductase (1997), Arch. Biochem. Biophys., 347, 221-228.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.2 NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.10.5.1 additional information construction of a chimeric enzyme of the human NQO2 with 43 amino acids from the carboxyl terminus of human DT-diaphorase. HNQO2-hDT43 still uses dihydronicotinamide riboside as an electron donor. The chimeric enzyme is inhibited by quercetin but not dicoumarol Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin) 0.01 mM, 25% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 4',5,7-trihydroxyflavone 0.01 mM, 61% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 alpha-naphthoflavone 0.01 mM, 75% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 beta-naphthoflavone 0.01 mM, 76% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 chrysin 0.01 mM, 100% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 galangin 0.01 mM, 100% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 morin 0.01 mM, 95% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 quercetin 0.01 mM, 100% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.10.5.1 2-hydroxyestradiol 0.01 mM, 17% inhibition Homo sapiens
1.10.5.1 4',5,7-trihydroxyflavone 0.01 mM, 61% inhibition Homo sapiens
1.10.5.1 4-hydroxyestradiol 0.01 mM, 18% inhibition Homo sapiens
1.10.5.1 4-hydroxyestrone 0.01 mM, 22% inhibition Homo sapiens
1.10.5.1 7,8-dihydroxyflavone 0.01 mM, 7% inhibition Homo sapiens
1.10.5.1 alpha-naphthoflavone 0.01 mM, 75% inhibition Homo sapiens
1.10.5.1 beta-naphthoflavone 0.01 mM, 76% inhibition Homo sapiens
1.10.5.1 chrysin 0.01 mM, complete inhibition Homo sapiens
1.10.5.1 dicoumarol 0.01 mM, 25% inhibition Homo sapiens
1.10.5.1 estradiol 0.01 mM, 13% inhibition Homo sapiens
1.10.5.1 flavone 0.01 mM, 14% inhibition Homo sapiens
1.10.5.1 galangin 0.01 mM, complete inhibition Homo sapiens
1.10.5.1 morin 0.01 mM, 95% inhibition Homo sapiens
1.10.5.1 quercetin 0.01 mM, complete inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.0023
-
 menadione recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 0.0027
-
 menadione recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
1.6.5.2 0.0047
-
 Methyl red recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 0.007
-
 oxidized 2,6-dichloroindophenol recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 0.012
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 0.018
-
 oxidized 2,6-dichloroindophenol recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
1.6.5.2 0.028
-
 reduced dihydronicotinamide riboside recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 0.028
-
 Methyl red recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
1.6.5.2 0.07
-
 reduced dihydronicotinamide riboside recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
1.6.5.2 0.26
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 1.37
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.10.5.1 0.0023
-
 menadione/3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens
1.10.5.1 0.0047
-
 Methyl red
-
 Homo sapiens
1.10.5.1 0.007
-
 2,6-dichlorophenolindophenol
-
 Homo sapiens
1.10.5.1 0.012
-
 3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens
1.10.5.1 0.019
-
 2,6-dichlorophenolindophenol chimeric enzyme of the human NQO2 with 43 amino acids from the carboxyl terminus of human DT-diaphorase Homo sapiens
1.10.5.1 0.028
-
 dihydronicotinamide riboside
-
 Homo sapiens
1.10.5.1 0.252
-
 NADH
-
 Homo sapiens
1.10.5.1 0.26
-
 CB 1954
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.2 26000
-
 2 * 26000, NAD(P)H:quinone acceptor oxidoreductase 2, SDS-PAGE Homo sapiens
1.6.5.2 50000
-
 NAD(P)H:quinone acceptor oxidoreductase 2, gel filtration Homo sapiens
1.10.5.1 26000
-
 2 * 26000, SDS-PAGE Homo sapiens
1.10.5.1 50000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Homo sapiens
-
 NAD(P)H:quinone acceptor oxidoreductase 2
-
1.10.5.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2 recombinant NAD(P)H:quinone acceptor oxidoreductase 2, affinity chromatography on Affi-gel blue Homo sapiens
1.10.5.1
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 methyl red + NAD(P)H
-
 Homo sapiens reduced methyl red + NAD(P)+
-
 ?
1.6.5.2 oxidized 2,6-dichlorophenolindophenol + NADH + H+
-
 Homo sapiens reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
1.6.5.2 oxidized 2,6-dichlorophenolindophenol + NADPH + H+
-
 Homo sapiens reduced 2,6-dichlorophenolindophenol + NADP+
-
 r
1.6.5.2 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium + NADPH + H+
-
 Homo sapiens reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium + NADP+
-
 r
1.6.5.2 oxidized methyl red + reduced dihydronicotinamide riboside + H+
-
 Homo sapiens reduced methyl red + oxidized dihydronicotinamide riboside
-
 r
1.10.5.1 dihydronicotinamide riboside + 2,6-dichlorophenolindophenol
-
 Homo sapiens nicotinamide riboside + reduced 2,6-dichlorophenolindophenol
-
 ?
1.10.5.1 dihydronicotinamide riboside + 3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens nicotinamide riboside + ?
-
 ?
1.10.5.1 dihydronicotinamide riboside + CB 1954
-
 Homo sapiens nicotinamide riboside + ?
-
 ?
1.10.5.1 dihydronicotinamide riboside + menadione/3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens nicotinamide riboside + ?
-
 ?
1.10.5.1 dihydronicotinamide riboside + methyl red
-
 Homo sapiens nicotinamide riboside + ?
-
 ?
1.10.5.1 additional information no activity with one-electron acceptors such as potassium ferricyanide Homo sapiens ?
-
 ?
1.10.5.1 NADH + 2,6-dichlorophenolindophenol
-
 Homo sapiens ?
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.5.2 dimer 2 * 26000, NAD(P)H:quinone acceptor oxidoreductase 2, SDS-PAGE Homo sapiens
1.10.5.1 dimer 2 * 26000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.10.5.1 NQO2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.2 0.01
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 0.333
-
 Methyl red recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
1.6.5.2 3.83
-
 Methyl red recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 6
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 11
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 38.3
-
 menadione recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 43.3
-
 reduced dihydronicotinamide riboside recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 46.7
-
 oxidized 2,6-dichlorophenolindophenol recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.6.5.2 517
-
 reduced dihydronicotinamide riboside recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
1.6.5.2 550
-
 menadione recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
1.6.5.2 600
-
 oxidized 2,6-dichlorophenolindophenol recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
1.10.5.1 2.6
-
 NADH
-
 Homo sapiens
1.10.5.1 3.83
-
 Methyl red
-
 Homo sapiens
1.10.5.1 6
-
 CB 1954
-
 Homo sapiens
1.10.5.1 11
-
 3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens
1.10.5.1 38.33
-
 menadione/3-(4,5-dimethylthiazaol-2-yl)-2,5-diphenyltetrazolium
-
 Homo sapiens
1.10.5.1 43.33
-
 dihydronicotinamide riboside
-
 Homo sapiens
1.10.5.1 46.67
-
 2,6-dichlorophenolindophenol
-
 Homo sapiens
1.10.5.1 91.8
-
 2,6-dichlorophenolindophenol chimeric enzyme of the human NQO2 with 43 amino acids from the carboxyl terminus of human DT-diaphorase Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FAD NAD(P)H:quinone acceptor oxidoreductase 2, contains 1 FAD per subunit Homo sapiens
1.6.5.2 nicotinamide riboside recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.10.5.1 FAD one prosthetic group per subunit Homo sapiens
1.10.5.1 NADH very poor electron donor Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.10.5.1 0.000021
-
 quercetin
-
 Homo sapiens