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Literature summary extracted from

  • Prochaska, H.J.; Talalay, P.
    Purification and characterization of two isofunctional forms of NAD(P)H: quinone reductase from mouse liver (1986), J. Biol. Chem., 261, 1372-1378.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.6.5.2 hydrophilic isoform in the presence of FAD, hanging drop diffusion, 10 mg/ml enzyme, in 200 mM potassium phosphate, 0.5 mM EDTA, 0.5 mM, FAD, pH 8.0, 45-50% saturation of ammonium sulfate Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin)
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.0000017
-
 FAD hydrophobic isoform Mus musculus
1.6.5.2 0.0000027
-
 FAD hydrophilic isoform Mus musculus
1.6.5.2 0.00196
-
 menadione hydrophilic isoform Mus musculus
1.6.5.2 0.00247
-
 menadione hydrophobic isoform Mus musculus
1.6.5.2 0.0581
-
 NADPH hydrophobic isoform Mus musculus
1.6.5.2 0.0643
-
 NADH hydrophilic isoform Mus musculus
1.6.5.2 0.066
-
 NADPH hydrophilic isoform Mus musculus
1.6.5.2 0.0881
-
 NADH hydrophobic isoform Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.2 cytosol
-
 Mus musculus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.2 30000
-
 2 * 30000, hydrophilic and hydrophobic isoform, SDS-PAGE Mus musculus
1.6.5.2 52000
-
 hydrophilic and hydrophobic isoforms, gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Mus musculus
-
 hydrophilic and hydrophobic isoform
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2 affinity chromatography on Cibacron blue-Agarose Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.2 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 1720
-
 hydrophilic isoform Mus musculus
1.6.5.2 1770
-
 hydrophobic isoform Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 1,4-naphthoquinone + NAD(P)H
-
 Mus musculus 1,4-naphthohydroquinone + NAD(P)+
-
 ?
1.6.5.2 2-methyl-1,4-naphthoquinone + NAD(P)H artificial acceptor, trivial name menadione Mus musculus 2-methyl-1,4-naphthoquinol + NAD(P)+
-
 ?
1.6.5.2 benzoquinone + 2 NAD(P)H + H+
-
 Mus musculus benzohydroquinone + 2 NAD(P)+
-
 ?
1.6.5.2 menadione + NADH + H+
-
 Mus musculus menadiol + NAD+
-
 r
1.6.5.2 NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Mus musculus NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.2 dimer 2 * 30000, hydrophilic and hydrophobic isoform, SDS-PAGE Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.5.2 0.000131
-
 dicoumarol hydrophilic isoform Mus musculus
1.6.5.2 0.000288
-
 dicoumarol hydrophobic isoform Mus musculus