EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.2 | p-hydroxymercuribenzoate | 0.1 mM, complete inhibition | Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.2 | 0.008 | - |
methemoglobin-ferrocyanide complex | - |
Oryctolagus cuniculus | |
1.6.2.2 | 0.01 | - |
ferricyanide | - |
Oryctolagus cuniculus | |
1.6.2.2 | 0.015 | - |
cytochrome b5 | - |
Oryctolagus cuniculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.6.2.2 | cytosol | - |
Oryctolagus cuniculus | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.6.2.2 | 25000 | - |
liver, cytosol, gel filtration, sucrose density gradient centrifugation | Oryctolagus cuniculus |
1.6.2.2 | 30000 | - |
enzyme from liver cytosol, gel filtration | Oryctolagus cuniculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.2 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.2.2 | - |
Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.6.2.2 | liver | - |
Oryctolagus cuniculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.6.2.2 | 1.69 | - |
methemoglobin-ferrocyanide reductases assay | Oryctolagus cuniculus |
EC Number | Storage Stability | Organism |
---|---|---|
1.6.2.2 | -20°C, several months, no loss of activity | Oryctolagus cuniculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.2 | 2 ferricytochrome b5 + NADH | specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
1.6.2.2 | 2 ferricytochrome b5 + NADH | additional acceptor: methemoglobin-ferrocyanide complex | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
1.6.2.2 | methemoglobin-ferrocyanide complex + NADH | - |
Oryctolagus cuniculus | reduced methemoglobin-ferrocyanide complex + NAD+ | - |
? | |
1.6.2.2 | NADH + ferricyanide | - |
Oryctolagus cuniculus | NAD+ + H+ + ferrocyanide | - |
r | |
1.6.2.2 | NADH + ferricytochrome b5 | - |
Oryctolagus cuniculus | NAD+ + H+ + ferrocytochrome b5 | - |
r |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.6.2.2 | 6.5 | - |
Tris-phosphate buffer | Oryctolagus cuniculus |
1.6.2.2 | 7 | - |
triethanolamine buffer | Oryctolagus cuniculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.2 | NADH | - |
Oryctolagus cuniculus |