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Literature summary extracted from

  • Montalbini, P.; Aguilar, M.; Pineda, M.
    Isolation and characterization of uricase from bean leaves and its comparison with uredospore enzymes (1999), Plant Sci., 147, 139-147.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 oxonate 0.025 mM 90% inhibition Phaseolus vulgaris
1.7.3.3 oxonate 0.025 mM 90% inhibition Uromyces phaseoli
1.7.3.3 oxonate 0.025 mM 90% inhibition Uromyces viciae-fabae
1.7.3.3 oxonate
-
 Vicia faba
1.7.3.3 xanthine 0.05 mM 60-75% inhibition Phaseolus vulgaris
1.7.3.3 xanthine 0.05 mM 60-75% inhibition Uromyces phaseoli
1.7.3.3 xanthine 0.05 mM 60-75% inhibition Uromyces viciae-fabae
1.7.3.3 xanthine
-
 Vicia faba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.3 0.015
-
 uric acid
-
 Phaseolus vulgaris
1.7.3.3 0.021
-
 uric acid
-
 Vicia faba
1.7.3.3 0.032
-
 uric acid
-
 Uromyces phaseoli
1.7.3.3 0.034
-
 uric acid
-
 Uromyces viciae-fabae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.3 120000 122000
-
 Vicia faba
1.7.3.3 124000
-
 PAGE Uromyces viciae-fabae
1.7.3.3 124000
-
 PAGE Uromyces phaseoli
1.7.3.3 128000
-
 PAGE Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.3 urate + O2 + H2O Phaseolus vulgaris
-
 5-hydroxyisourate + H2O2
-
 ?
1.7.3.3 urate + O2 + H2O Vicia faba
-
 5-hydroxyisourate + H2O2
-
 ?
1.7.3.3 urate + O2 + H2O Uromyces viciae-fabae
-
 5-hydroxyisourate + H2O2
-
 ?
1.7.3.3 urate + O2 + H2O Uromyces phaseoli
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Phaseolus vulgaris
-
-
-
1.7.3.3 Uromyces phaseoli
-
-
-
1.7.3.3 Uromyces viciae-fabae
-
-
-
1.7.3.3 Vicia faba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.3
-
 Phaseolus vulgaris
1.7.3.3
-
 Uromyces viciae-fabae
1.7.3.3
-
 Uromyces phaseoli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.3.3 leaf
-
 Phaseolus vulgaris
-
1.7.3.3 leaf
-
 Vicia faba
-
1.7.3.3 uredospore
-
 Uromyces viciae-fabae
-
1.7.3.3 uredospore
-
 Uromyces phaseoli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.3.3 1.87
-
-
 Phaseolus vulgaris
1.7.3.3 2.04
-
-
 Uromyces viciae-fabae
1.7.3.3 3.11
-
-
 Uromyces phaseoli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Phaseolus vulgaris 5-hydroxyisourate + H2O2
-
 ?
1.7.3.3 urate + O2 + H2O
-
 Vicia faba 5-hydroxyisourate + H2O2
-
 ?
1.7.3.3 urate + O2 + H2O
-
 Uromyces viciae-fabae 5-hydroxyisourate + H2O2
-
 ?
1.7.3.3 urate + O2 + H2O
-
 Uromyces phaseoli 5-hydroxyisourate + H2O2
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.3.3 8 9
-
 Uromyces phaseoli
1.7.3.3 8.5 9.1
-
 Uromyces viciae-fabae
1.7.3.3 8.6 9.5
-
 Vicia faba
1.7.3.3 9 9.7
-
 Phaseolus vulgaris