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Literature summary extracted from

  • Ohe, T.; Watanabe, Y.
    Purification and properties of urate oxidase from Streptomyces cyanogenus (1981), J. Biochem., 89, 1769-1776.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 2-Hydroxypurine
-
 Streptomyces cyanogenus
1.7.3.3 CN-
-
 Streptomyces cyanogenus
1.7.3.3 iodoacetate low effect Streptomyces cyanogenus
1.7.3.3 p-chloromercuribenzoate slight effect Streptomyces cyanogenus
1.7.3.3 Urate not Streptomyces cyanogenus
1.7.3.3 xanthine
-
 Streptomyces cyanogenus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.3 0.099
-
 uric acid
-
 Streptomyces cyanogenus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.3.3 cell associated
-
 Streptomyces cyanogenus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.3.3 copper enzyme contains 0.15 mol of copper Streptomyces cyanogenus
1.7.3.3 Iron enzyme contains less than 0.05 mol of iron Streptomyces cyanogenus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.3 32000
-
 alpha3, 3 * 32000, SDS-PAGE Streptomyces cyanogenus
1.7.3.3 109000
-
 gel filtration Streptomyces cyanogenus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.3 urate + O2 + H2O Streptomyces cyanogenus
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Streptomyces cyanogenus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.3
-
 Streptomyces cyanogenus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.3.3 12.3
-
-
 Streptomyces cyanogenus

Storage Stability

EC Number Storage Stability Organism
1.7.3.3 4°C, 20 mM phosphate buffer, pH 7.8, for at least 1 month Streptomyces cyanogenus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Streptomyces cyanogenus 5-hydroxyisourate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.3.3 trimer alpha3, 3 * 32000, SDS-PAGE Streptomyces cyanogenus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.3.3 35
-
-
 Streptomyces cyanogenus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.3.3 50
-
 pH 7.8, 10 min, stable below Streptomyces cyanogenus
1.7.3.3 60
-
 pH 7.8, 10 min, 50% loss of activity Streptomyces cyanogenus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.3 8 8.5 borate and phosphate buffer Streptomyces cyanogenus
1.7.3.3 9.5
-
 glycine buffer Streptomyces cyanogenus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.3.3 6 11 35°C, 1 h, stable Streptomyces cyanogenus