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Literature summary extracted from
- Purification and properties of thiosulfate dehydrogenase from Acidithiobacillus thiooxidans JCM7814 (2001), Biosci. Biotechnol. Biochem., 65, 102-108.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.99.B2 | 0.81 | - |
thiosulfate | - |
Acidithiobacillus thiooxidans |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.99.B2 | periplasm | - |
Acidithiobacillus thiooxidans | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 27000 | - |
gel filtration | Acidithiobacillus thiooxidans |
| 1.8.99.B2 | 27900 | - |
1 * 27900, SDS-PAGE | Acidithiobacillus thiooxidans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.99.B2 | Acidithiobacillus thiooxidans | - |
JCM7814 | - |
| 1.8.99.B2 | Acidithiobacillus thiooxidans JCM 7814 | - |
JCM7814 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.99.B2 | using chromatography on Phenyl-Sepharose column, CM-cellulose column and hydroxylapatite column | Acidithiobacillus thiooxidans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 0.06 | - |
- |
Acidithiobacillus thiooxidans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.99.B2 | thiosulfate + oxidized acceptor | the activity does not couple with mammalian cytochrome c as electron acceptor | Acidithiobacillus thiooxidans | tetrathionate + reduced acceptor | - |
? | |
| 1.8.99.B2 | thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Acidithiobacillus thiooxidans | tetrathionate + reduced acceptor | - |
? | |
| 1.8.99.B2 | thiosulfate + oxidized acceptor | the activity does not couple with mammalian cytochrome c as electron acceptor | Acidithiobacillus thiooxidans JCM 7814 | tetrathionate + reduced acceptor | - |
? | |
| 1.8.99.B2 | thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Acidithiobacillus thiooxidans JCM 7814 | tetrathionate + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.99.B2 | monomer | 1 * 27900, SDS-PAGE | Acidithiobacillus thiooxidans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 35 | - |
- |
Acidithiobacillus thiooxidans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 45 | - |
stable up to | Acidithiobacillus thiooxidans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 3.5 | - |
- |
Acidithiobacillus thiooxidans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.B2 | 5 | 7 | - |
Acidithiobacillus thiooxidans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.B2 | heme c | - |
Acidithiobacillus thiooxidans | |
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