Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Charles, A.M.; Suzuki, I.
    Purification and properties of sulfite:cytochrome c oxidoreductase from Thiobacillus novellus (1966), Biochim. Biophys. Acta, 128, 522-534.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.8.2.1 stable to repeated freezing and thawing Starkeya novella

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.1 AMP 0.01 mM, 25% inhibition, activity with ferricyanide Starkeya novella
1.8.2.1 EDTA 0.005 mM, 20% inhibition Starkeya novella
1.8.2.1 HgCl2 0.667 mM, 56% inhibition Starkeya novella
1.8.2.1 additional information CaCl2, MgSO4, ZnSO4, CuSO4, FeCl3, NiCl2 and Na2MoO4 inhibits the reaction by 70% at 0.267 mM, this effect is apparently due to the non-enzymatic oxidation of sulfite with air Starkeya novella
1.8.2.1 NaAsO2 31% inhibition Starkeya novella
1.8.2.1 NaCl competitive with respect to sulfite Starkeya novella
1.8.2.1 NaF 0.01 mM, 17.5% inhibition Starkeya novella
1.8.2.1 NEM 0.667 mM, 30% loss of activity Starkeya novella
1.8.2.1 p-hydroxymercuribenzoate 0.667 mM, 86% loss of activity, inhibition is completely reversed by GSH Starkeya novella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.1 0.002
-
 sulfite pH 6.5, reaction with cytochrome c Starkeya novella
1.8.2.1 0.02
-
 ferricyanide pH 8.0 Starkeya novella
1.8.2.1 0.04
-
 sulfite pH 8.0, reaction with cytochrome c Starkeya novella

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.2.1 sulfite + ferricytochrome c + H2O Starkeya novella oxidative phosphorylation is coupled to sulfite oxidation with a low P/O ratio sulfate + ferrocytochrome c + H+
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.8.2.1 Ethanol 50%, significant loss of activity Starkeya novella

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.1 Starkeya novella
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.1
-
 Starkeya novella

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.1 139.5
-
 reaction with ferricyanide Starkeya novella

Storage Stability

EC Number Storage Stability Organism
1.8.2.1 -20°C, retains high activity for more than 6 months Starkeya novella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.1 sulfite + ferricyanide + H2O
-
 Starkeya novella sulfate + ferrocyanide + H+
-
 ?
1.8.2.1 sulfite + ferricytochrome c + H2O specific for sulfite Starkeya novella sulfate + ferrocytochrome c + H+
-
 ?
1.8.2.1 sulfite + ferricytochrome c + H2O oxidative phosphorylation is coupled to sulfite oxidation with a low P/O ratio Starkeya novella sulfate + ferrocytochrome c + H+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.2.1 55
-
 complete loss of activity after 5 min, 25% loss of activity after 5 min in presence of 0.05 M sodium sulfite Starkeya novella
1.8.2.1 60
-
 1 min, complete loss of activity Starkeya novella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.1 8
-
 potassium phosphate buffer Starkeya novella

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.2.1 4.5
-
 NaCl
-
 Starkeya novella