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Literature summary extracted from
- Lipoamide dehydrogenase from Streptomyces seoulensis: biochemical and genetic properties (1998), Biochim. Biophys. Acta, 1388, 405-418.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.4 | - |
Streptomyces seoulensis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.4 | NAD+ | - |
Streptomyces seoulensis |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.4 | 0.37 | - |
Lipoamide | - |
Streptomyces seoulensis | |
| 1.8.1.4 | 0.4 | - |
1,4-benzoquinone | - |
Streptomyces seoulensis | |
| 1.8.1.4 | 2.15 | - |
lipoic acid | - |
Streptomyces seoulensis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.4 | 54000 | - |
2 * 54000, SDS-PAGE | Streptomyces seoulensis |
| 1.8.1.4 | 110000 | - |
gel filtration | Streptomyces seoulensis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.4 | Streptomyces seoulensis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.4 | - |
Streptomyces seoulensis |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.1.4 | protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ | ping-pong mechanism | Streptomyces seoulensis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.4 | 0.311 | - |
- |
Streptomyces seoulensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.4 | 1,4-benzoquinone + NADH | - |
Streptomyces seoulensis | 1,4-benzoquinol + NAD+ | - |
? | |
| 1.8.1.4 | 2 ferricyanide + NADH | - |
Streptomyces seoulensis | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| 1.8.1.4 | 2-hydroxy-1,4-benzoquinone + NADH | - |
Streptomyces seoulensis | 2-hydroxy-1,4-benzoquinol + NAD+ | - |
? | |
| 1.8.1.4 | 2-methyl-1,4-benzoquinone + NADH | - |
Streptomyces seoulensis | 2-methyl-1,4-benzoquinol + NAD+ | - |
? | |
| 1.8.1.4 | dihydrolipoamide + NAD+ | - |
Streptomyces seoulensis | lipoamide + NADH | - |
? | |
| 1.8.1.4 | lipoic acid + NADH + H+ | - |
Streptomyces seoulensis | dihydrolipoic acid + NAD+ | - |
r | |
| 1.8.1.4 | naphthoquinone + NADH | - |
Streptomyces seoulensis | 1,4-naphthoquinol + NAD+ | - |
? | |
| 1.8.1.4 | oxidized 2,6-dichlorophenolindophenol + NADH | - |
Streptomyces seoulensis | ? + NAD+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.4 | dimer | 2 * 54000, SDS-PAGE | Streptomyces seoulensis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.4 | 70 | - |
lipoic acid | - |
Streptomyces seoulensis | |
| 1.8.1.4 | 88 | - |
Lipoamide | - |
Streptomyces seoulensis | |
| 1.8.1.4 | 108 | - |
1,4-benzoquinone | - |
Streptomyces seoulensis | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.4 | FAD | contains 1 FAD per subunit | Streptomyces seoulensis | |
| 1.8.1.4 | NAD+ | - |
Streptomyces seoulensis | |
| 1.8.1.4 | NADH | - |
Streptomyces seoulensis | |
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