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Literature summary extracted from

  • Conner, M.; Krell, T.; Lindsay, J.G.
    Identification and purification of a distinct dihydrolipoamide dehydrogenase from pea chloroplasts (1996), Planta, 200, 195-202.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 NADH chloroplastic enzyme is more susceptible to product inhibition than the mitochondrial enzyme Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.1 0.11 NAD+ chloroplastic enzyme Pisum sativum
1.8.1.4 0.62 0.64 NAD+ mitochondrial enzyme Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.4 chloroplast
-
 Pisum sativum 9507
-
1.8.1.4 mitochondrion
-
 Pisum sativum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 49757
-
 x * 49757, mitochondrial enzyme, electrospray mass spectrometry Pisum sativum
1.8.1.4 52000
-
 x * 52000, enzyme from chloroplast, SDS-PAGE Pisum sativum
1.8.1.4 52614
-
 x * 52614, enzyme from chloroplast, electrospray mass spectrometry Pisum sativum
1.8.1.4 56000
-
 x * 56000, mitochondrial enzyme, SDS-PAGE Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Escherichia coli
-
-
-
1.8.1.4 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.4 leaf
-
 Pisum sativum
-
1.8.1.4 root
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.4 62
-
 enzyme from chloroplast Pisum sativum
1.8.1.4 295
-
 mitochondrial enzyme Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Escherichia coli lipoamide + NADH
-
 ?
1.8.1.4 dihydrolipoamide + NAD+
-
 Pisum sativum lipoamide + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.4 ?
-
 Escherichia coli
1.8.1.4 ? x * 56000, mitochondrial enzyme, SDS-PAGE Pisum sativum
1.8.1.4 ? x * 49757, mitochondrial enzyme, electrospray mass spectrometry Pisum sativum
1.8.1.4 ? x * 52000, enzyme from chloroplast, SDS-PAGE Pisum sativum
1.8.1.4 ? x * 52614, enzyme from chloroplast, electrospray mass spectrometry Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 NAD+
-
 Escherichia coli
1.8.1.4 NAD+
-
 Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.4 0.02
-
 NADH chloroplastic enzyme Pisum sativum
1.8.1.4 0.11
-
 NADH mitochondrial enzyme Pisum sativum