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Literature summary extracted from

  • Borges, A.; Hawkins, C.F.; Packman, L.C.; Perham, R.N.
    Cloning and sequence analysis of the genes encoding the dihydrolipoamide acetyltransferase and dihydrolipoamide dehydrogenase components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus (1990), Eur. J. Biochem., 194, 95-102.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 dihydrolipoamide dehydrogenase component of the pyruvate dehydrogenase multienzyme complex, expression in Escherichia coli Geobacillus stearothermophilus
2.3.1.12
-
 Geobacillus stearothermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.12 46265
-
 x * 46265, calculation from nucleotide sequence Geobacillus stearothermophilus
2.3.1.12 64260
-
 calculation from nucleotide sequence Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA Geobacillus stearothermophilus
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Geobacillus stearothermophilus
-
 dihydrolipoamide dehydrogenase component of the pyruvate dehydrogenase multienzyme complex
-
2.3.1.12 Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Geobacillus stearothermophilus lipoamide + NADH
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Geobacillus stearothermophilus S-acetyldihydrolipoamide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.12 polymer x * 46265, calculation from nucleotide sequence Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 NAD+
-
 Geobacillus stearothermophilus