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Substrate phosphorylation in Chlorobium vibrioforme f. sp. thiosulfatophilum

Khanna, S.; Nicholas, D.J.D.; J. Gen. Microbiol. 129, 1365-1370 (1983)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.7.5
very labile in crude extract, partially stabilized by addition of EDTA and 2-mercaptoethanol
Prosthecochloris vibrioformis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.99.2
AMP
above 2 mM
Prosthecochloris vibrioformis
1.8.99.2
iodoacetamide
5 mM, 54% inhibition
Prosthecochloris vibrioformis
1.8.99.2
NEM
5 mM, 49% inhibition
Prosthecochloris vibrioformis
1.8.99.2
PCMB
1 mM, 30% inhibition
Prosthecochloris vibrioformis
2.7.7.5
iodoacetamide
-
Prosthecochloris vibrioformis
2.7.7.5
N-ethylmaleimide
very potent inhibitor, partially reversible by thiol-containing reagents
Prosthecochloris vibrioformis
2.7.7.5
p-chloromercuribenzoate
very potent inhibitor, partially reversible by thiol-containing reagents
Prosthecochloris vibrioformis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.99.2
0.13
-
AMP
-
Prosthecochloris vibrioformis
1.8.99.2
0.17
-
sulfite
-
Prosthecochloris vibrioformis
1.8.99.2
0.25
-
adenylyl sulfate
-
Prosthecochloris vibrioformis
1.8.99.2
4.2
-
ferricyanide
-
Prosthecochloris vibrioformis
2.7.7.5
0.25
-
adenosine 5'-phosphosulfate
-
Prosthecochloris vibrioformis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.99.2
Iron
contains 4-6 mol of non-haem iron per mol of enzyme
Prosthecochloris vibrioformis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.99.2
180000
-
gel filtration
Prosthecochloris vibrioformis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.5
phosphate + adenosine 5'-phosphosulfate
Prosthecochloris vibrioformis
-
ADP + SO42-
-
Prosthecochloris vibrioformis
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.99.2
Prosthecochloris vibrioformis
-
f. sp. thiosulfatophilum
-
2.7.7.5
Prosthecochloris vibrioformis
-
f. sp. thiosulfatophilum, strain NCIB 8346
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.99.2
-
Prosthecochloris vibrioformis
2.7.7.5
-
Prosthecochloris vibrioformis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.99.2
13.15
-
-
Prosthecochloris vibrioformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.99.2
AMP + sulfite + ferricyanide
-
393933
Prosthecochloris vibrioformis
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
GMP + sulfite + ferricyanide
54% of the activity with AMP
393933
Prosthecochloris vibrioformis
guanylyl sulfate + ferrocyanide
-
-
-
?
2.7.7.5
phosphate + adenosine 5'-phosphosulfate
-
393933
Prosthecochloris vibrioformis
ADP + SO42-
-
393933
Prosthecochloris vibrioformis
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.99.2
8
-
-
Prosthecochloris vibrioformis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.99.2
FAD
-
Prosthecochloris vibrioformis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.99.2
FAD
-
Prosthecochloris vibrioformis
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.5
very labile in crude extract, partially stabilized by addition of EDTA and 2-mercaptoethanol
Prosthecochloris vibrioformis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.99.2
AMP
above 2 mM
Prosthecochloris vibrioformis
1.8.99.2
iodoacetamide
5 mM, 54% inhibition
Prosthecochloris vibrioformis
1.8.99.2
NEM
5 mM, 49% inhibition
Prosthecochloris vibrioformis
1.8.99.2
PCMB
1 mM, 30% inhibition
Prosthecochloris vibrioformis
2.7.7.5
iodoacetamide
-
Prosthecochloris vibrioformis
2.7.7.5
N-ethylmaleimide
very potent inhibitor, partially reversible by thiol-containing reagents
Prosthecochloris vibrioformis
2.7.7.5
p-chloromercuribenzoate
very potent inhibitor, partially reversible by thiol-containing reagents
Prosthecochloris vibrioformis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.99.2
0.13
-
AMP
-
Prosthecochloris vibrioformis
1.8.99.2
0.17
-
sulfite
-
Prosthecochloris vibrioformis
1.8.99.2
0.25
-
adenylyl sulfate
-
Prosthecochloris vibrioformis
1.8.99.2
4.2
-
ferricyanide
-
Prosthecochloris vibrioformis
2.7.7.5
0.25
-
adenosine 5'-phosphosulfate
-
Prosthecochloris vibrioformis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.99.2
Iron
contains 4-6 mol of non-haem iron per mol of enzyme
Prosthecochloris vibrioformis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.99.2
180000
-
gel filtration
Prosthecochloris vibrioformis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.5
phosphate + adenosine 5'-phosphosulfate
Prosthecochloris vibrioformis
-
ADP + SO42-
-
Prosthecochloris vibrioformis
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.99.2
-
Prosthecochloris vibrioformis
2.7.7.5
-
Prosthecochloris vibrioformis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.99.2
13.15
-
-
Prosthecochloris vibrioformis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.99.2
AMP + sulfite + ferricyanide
-
393933
Prosthecochloris vibrioformis
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
GMP + sulfite + ferricyanide
54% of the activity with AMP
393933
Prosthecochloris vibrioformis
guanylyl sulfate + ferrocyanide
-
-
-
?
2.7.7.5
phosphate + adenosine 5'-phosphosulfate
-
393933
Prosthecochloris vibrioformis
ADP + SO42-
-
393933
Prosthecochloris vibrioformis
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.99.2
8
-
-
Prosthecochloris vibrioformis