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Literature summary extracted from
- Adenylylsulfate reductase in some new sulfate-reducing bacteria (1984), Arch. Microbiol., 137, 145-150.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.2 | AMP | inhibits reaction with cytochrome c above 0.1 mM, inhibits reaction with ferricyanide above 1.6 mM | Desulfobulbus propionicus |  |
| 1.8.99.2 | AMP | - |
Desulfovibrio vulgaris | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.99.2 | 0.021 | - |
cytochrome c | - |
Desulfobulbus propionicus | |
| 1.8.99.2 | 0.071 | - |
sulfite | reaction with cytochrome c | Desulfobulbus propionicus | |
| 1.8.99.2 | 0.089 | - |
ferricyanide | - |
Desulfobulbus propionicus | |
| 1.8.99.2 | 0.09 | - |
AMP | reaction with ferricyanide | Desulfobulbus propionicus | |
| 1.8.99.2 | 0.091 | - |
AMP | reaction with cytochrome c | Desulfobulbus propionicus | |
| 1.8.99.2 | 1.3 | - |
sulfite | reaction with ferricyanide | Desulfobulbus propionicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.2 | Iron | - |
Desulfovibrio vulgaris | |
| 1.8.99.2 | Iron | contains 8 mol of iron per mol of enzyme | Desulfobulbus propionicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.2 | 175000 | - |
gel filtration | Desulfobulbus propionicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.99.2 | Desulfobacter postgatei | - |
- |
- |
| 1.8.99.2 | Desulfobulbus propionicus | - |
- |
- |
| 1.8.99.2 | Desulfococcus multivorans | - |
- |
- |
| 1.8.99.2 | Desulfosarcina variabilis | - |
- |
- |
| 1.8.99.2 | Desulfovibrio vulgaris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.99.2 | - |
Desulfobulbus propionicus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.2 | 8.93 | - |
- |
Desulfobulbus propionicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.8.99.2 | -18°C, 88-90% loss of activity after 1 week, 68% loss of activity after 16 days, in presence of 50% glycerol, 46% loss of activity after 16 days | Desulfobulbus propionicus |
| 1.8.99.2 | 4°C, more than 20% loss of activity after 8 h | Desulfobulbus propionicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.99.2 | ADP + sulfite + ferricyanide | - |
Desulfobulbus propionicus | ? + ferrocyanide | - |
? | |
| 1.8.99.2 | AMP + sulfite + cytochrome c | - |
Desulfovibrio vulgaris | adenylyl sulfate + ? | - |
? | |
| 1.8.99.2 | AMP + sulfite + cytochrome c | reaction with horse heart cytochrome c, no activity with cytochrome c from Candida krusei | Desulfobulbus propionicus | adenylyl sulfate + ? | - |
? | |
| 1.8.99.2 | AMP + sulfite + ferricyanide | - |
Desulfovibrio vulgaris | adenylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | AMP + sulfite + ferricyanide | - |
Desulfobulbus propionicus | adenylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | ATP + sulfite + ferricyanide | 12.8% of the activity with AMP | Desulfobulbus propionicus | ? + ferrocyanide | - |
? | |
| 1.8.99.2 | CMP + sulfite + ferricyanide | - |
Desulfovibrio vulgaris | cytidylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | CMP + sulfite + ferricyanide | 7.6% of the activity with AMP | Desulfobulbus propionicus | cytidylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | deoxyAMP + sulfite + ferricyanide | 82.7% of the activity with AMP | Desulfobulbus propionicus | deoxyadenylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | GMP + sulfite + ferricyanide | - |
Desulfovibrio vulgaris | guanylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | GMP + sulfite + ferricyanide | 29.4% of the activity with AMP | Desulfobulbus propionicus | guanylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | UMP + sulfite + ferricyanide | - |
Desulfovibrio vulgaris | uridylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | UMP + sulfite + ferricyanide | 7.6% of the activity with AMP | Desulfobulbus propionicus | uridylyl sulfate + ferrocyanide | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.2 | 65 | - |
5 min, complete loss of activity | Desulfobulbus propionicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.2 | 7.7 | - |
reaction with ferricyanide | Desulfobulbus propionicus |
| 1.8.99.2 | 8.8 | - |
reaction with cytochrome c | Desulfobulbus propionicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.2 | flavin | contains 1 mol of flavin per mol of enzyme | Desulfobulbus propionicus | |
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