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Literature summary extracted from

  • Steenkamp, D.J.; Mallinson, J.
    Trimethylamine dehydrogenase from a methylotrophic bacterium. I. Isolation and steady-state kinetics (1976), Biochim. Biophys. Acta, 429, 7005-719.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.8.2 acetaldehyde noncompetitive inhibition with respect to diethylamine, uncompetitive inhibition with respect to phenazine methosulfate Methylophilus methylotrophus
1.5.8.2 Diethylamine noncompetitive product inhibition with respect to phenazine methosulfate Methylophilus methylotrophus
1.5.8.2 ethylamine noncompetitive product inhibition with respect to phenazine methosulfate Methylophilus methylotrophus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.8.2 146800
-
 sedimentation equilibrium ultracentrifugation Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.2 trimethylamine + H2O + electron acceptor Methylophilus methylotrophus ping-ping reaction mechanism dimethylamine + formaldehyde + reduced electron acceptor
-
 ?
1.5.8.2 trimethylamine + H2O + electron acceptor Methylophilus methylotrophus W3A1 ping-ping reaction mechanism dimethylamine + formaldehyde + reduced electron acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.2 Methylophilus methylotrophus
-
-
-
1.5.8.2 Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.2 to homogeneity, chromatography steps Methylophilus methylotrophus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.2 cell culture
-
 Methylophilus methylotrophus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.8.2 1.07
-
-
 Methylophilus methylotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.2 diethylamine + H2O + phenazine methosulfate
-
 Methylophilus methylotrophus ethylamine + acetaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 diethylamine + H2O + phenazine methosulfate
-
 Methylophilus methylotrophus W3A1 ethylamine + acetaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
 Methylophilus methylotrophus dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.8.2 trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
 Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.8.2 trimethylamine + H2O + electron acceptor ping-ping reaction mechanism Methylophilus methylotrophus dimethylamine + formaldehyde + reduced electron acceptor
-
 ?
1.5.8.2 trimethylamine + H2O + electron acceptor ping-ping reaction mechanism Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced electron acceptor
-
 ?
1.5.8.2 trimethylamine + H2O + phenazine methosulfate
-
 Methylophilus methylotrophus dimethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 trimethylamine + H2O + phenazine methosulfate
-
 Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.8.2 dimer non-identical subunits of 70000-80000, SDS-PAGE Methylophilus methylotrophus