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Literature summary extracted from

  • Basran, J.; Sutcliffe, M.J.; Hille, R.; Scrutton, N.S.
    Reductive half-reaction of the H172Q mutant of trimethylamine dehydrogenase: Evidence against a carbanion mechanism and assignment of kinetically influential ionizations in the enzyme-substrate complex (1999), Biochem. J., 341, 307-314.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.8.2 expression in Escherichia coli Methylophilus methylotrophus

Protein Variants

EC Number Protein Variants Comment Organism
1.5.8.2 H172Q no change of turnover, but 25fold increase of Km-value Methylophilus methylotrophus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.8.2 trimethylamine
-
 Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein Methylophilus methylotrophus
-
 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein Methylophilus methylotrophus W3A1
-
 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.2 Methylophilus methylotrophus
-
-
-
1.5.8.2 Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.2 to homogeneity, recombinant enzyme Methylophilus methylotrophus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.2 cell culture
-
 Methylophilus methylotrophus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein
-
 Methylophilus methylotrophus dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?
1.5.8.2 trimethylamine + H2O + electron transferring flavoprotein
-
 Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced electron transferring flavoprotein
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.8.2 FMN
-
 Methylophilus methylotrophus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.8.2 0.75
-
 trimethylamine
-
 Methylophilus methylotrophus